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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-3-1
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pubmed:abstractText |
We investigated the roles of netrin-1 and slit-2 in regulation and navigation of dopamine (DA) axon growth using an explant culture preparation of embryonic ventral midbrain (embryonic day 14) and a co-culture system. We found that netrin-1 protein significantly enhanced DA axonal outgrowth and promoted DA axonal outgrowth in a co-culture system of netrin-1 expressing cells. Such effects were mediated by the receptor DCC as demonstrated by antibody perturbation of the DCC receptor. In contrast, slit-2 inhibited DA neuron extensions and repelled DA neurite growth. These slit-2 activities required robo receptors since the reduced neurite extension was abolished by addition of excess robo receptors. In this system, netrin-1 stimulated and slit-2 opposed DA neurite growth. Such regulation may be important for DA axonal maintenance, regeneration, and phenotypic target recognition.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DCC protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/G Protein-Coupled...,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/ROBO2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Slit homolog 2 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/netrin-1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1044-7431
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
547-55
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pubmed:dateRevised |
2009-10-1
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pubmed:meshHeading |
pubmed-meshheading:15737744-Animals,
pubmed-meshheading:15737744-Cell Adhesion Molecules,
pubmed-meshheading:15737744-Cell Differentiation,
pubmed-meshheading:15737744-Cell Line,
pubmed-meshheading:15737744-Cells, Cultured,
pubmed-meshheading:15737744-Dopamine,
pubmed-meshheading:15737744-G Protein-Coupled Inwardly-Rectifying Potassium Channels,
pubmed-meshheading:15737744-Growth Cones,
pubmed-meshheading:15737744-Growth Inhibitors,
pubmed-meshheading:15737744-Growth Substances,
pubmed-meshheading:15737744-Humans,
pubmed-meshheading:15737744-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:15737744-Mesencephalon,
pubmed-meshheading:15737744-Nerve Growth Factors,
pubmed-meshheading:15737744-Nerve Tissue Proteins,
pubmed-meshheading:15737744-Neurites,
pubmed-meshheading:15737744-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:15737744-Rats,
pubmed-meshheading:15737744-Rats, Sprague-Dawley,
pubmed-meshheading:15737744-Receptors, Cell Surface,
pubmed-meshheading:15737744-Receptors, Immunologic,
pubmed-meshheading:15737744-Substantia Nigra,
pubmed-meshheading:15737744-Tumor Suppressor Proteins,
pubmed-meshheading:15737744-Tyrosine 3-Monooxygenase
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pubmed:year |
2005
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pubmed:articleTitle |
Netrin-1 and slit-2 regulate and direct neurite growth of ventral midbrain dopaminergic neurons.
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pubmed:affiliation |
Neuroregeneration Laboratories, Mailman Research Center, Harvard Medical School/McLean Hospital, 115 Mill Street, Belmont, MA 02478, USA. llin@mclean.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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