Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-3-1
pubmed:abstractText
The presence of multiple axon growth inhibitors may partly explain why central nervous system axons are generally incapable of regenerating after injury. Using RNA interference (RNAi) in dorsal root ganglia neurons (DRGN), we demonstrate siRNA-mediated silencing of components of the inhibitory signalling cascade, including p75NTR, NgR and Rho-A mRNA, of 70%, 100% and 100% of the relevant protein, respectively, while changes in neither protein levels nor cellular immunoreactivity were detected using the relevant scrambled siRNA control sequences. Importantly, after 48 h in culture after siRNA-mediated knockdown of Rho-A, neurite outgrowth was enhanced by 30% compared to that after p75NTR and 50% after NgR silencing. By 3 days, a 5-, 3.5- and 6.5-fold increase in betaIII-tubulin protein levels were observed compared to controls without siRNA after knockdown of p75NTR, NgR and Rho-A, respectively. Together, these results suggest that Rho-A knockdown might be the most effective target for a disinhibition strategy to promote CNS axon regeneration in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Myelin Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Nerve Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Peptide, http://linkedlifedata.com/resource/pubmed/chemical/Rtn4r protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Tubulin, http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1044-7431
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
509-23
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15737741-Animals, pubmed-meshheading:15737741-Cells, Cultured, pubmed-meshheading:15737741-Down-Regulation, pubmed-meshheading:15737741-Feedback, Physiological, pubmed-meshheading:15737741-GPI-Linked Proteins, pubmed-meshheading:15737741-Ganglia, Spinal, pubmed-meshheading:15737741-Myelin Proteins, pubmed-meshheading:15737741-Nerve Growth Factors, pubmed-meshheading:15737741-Nerve Regeneration, pubmed-meshheading:15737741-Neurites, pubmed-meshheading:15737741-RNA, Small Interfering, pubmed-meshheading:15737741-Rats, pubmed-meshheading:15737741-Rats, Sprague-Dawley, pubmed-meshheading:15737741-Receptor, Nerve Growth Factor, pubmed-meshheading:15737741-Receptors, Cell Surface, pubmed-meshheading:15737741-Receptors, Nerve Growth Factor, pubmed-meshheading:15737741-Receptors, Peptide, pubmed-meshheading:15737741-Tubulin, pubmed-meshheading:15737741-Up-Regulation, pubmed-meshheading:15737741-rhoA GTP-Binding Protein
pubmed:year
2005
pubmed:articleTitle
Disinhibition of neurotrophin-induced dorsal root ganglion cell neurite outgrowth on CNS myelin by siRNA-mediated knockdown of NgR, p75NTR and Rho-A.
pubmed:affiliation
Molecular Neuroscience Group, Department of Medicine, University of Birmingham, 3rd Floor Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Edgbaston, Birmingham B15 2TH, UK. z.ahmed.1@bham.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't