Linked Life Data

15735688

Source:http://linkedlifedata.com/resource/pubmed/id/15735688

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2005-7-7
pubmed:abstractText
Interleukin-2 (IL-2) mediates cell cycle progression and antiapoptosis in human T cells via several signal transduction pathways. The Tax protein of the human T-cell leukemia virus type I (HTLV-1) deregulates cell growth and alters the role of IL-2 in infected cells. However, Tax-immortalized cells stay dependent on IL-2, suggesting that events besides HTLV-1 gene expression are required for leukemia to develop. Here, IL-2-dependent and -independent events were analysed in a human T cell line immortalized by Tax. These studies show that, of the signaling pathways evaluated, only STAT5 remains dependent. Microarray analyses revealed several genes, including il-5, il-9 and il-13, are uniquely upregulated by IL-2 in the presence of Tax. Bioinformatics and supporting molecular biology show that some of these genes are STAT5 targets, explaining their IL-2 upregulation. These results suggest that IL-2 and viral proteins work together to induce gene expression, promoting the hypothesis that deregulation via the constitutive activation of STAT5 may lead to the IL-2-independent phenotype of HTLV-1-transformed cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4624-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15735688-Cell Culture Techniques, pubmed-meshheading:15735688-Cytokines, pubmed-meshheading:15735688-DNA-Binding Proteins, pubmed-meshheading:15735688-Gene Expression Regulation, Neoplastic, pubmed-meshheading:15735688-Gene Products, tax, pubmed-meshheading:15735688-Human T-lymphotropic virus 1, pubmed-meshheading:15735688-Humans, pubmed-meshheading:15735688-Interleukin-2, pubmed-meshheading:15735688-Milk Proteins, pubmed-meshheading:15735688-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:15735688-Phenotype, pubmed-meshheading:15735688-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15735688-STAT5 Transcription Factor, pubmed-meshheading:15735688-Signal Transduction, pubmed-meshheading:15735688-Thymus Gland, pubmed-meshheading:15735688-Trans-Activators
pubmed:year
2005
pubmed:articleTitle
IL-2- and STAT5-regulated cytokine gene expression in cells expressing the Tax protein of HTLV-1.
pubmed:affiliation
Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural