| pubmed-article:15734732 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0003402 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1417701 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0205160 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1412729 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1136197 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1426958 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0600508 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C1522538 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:15734732 | lifeskim:mentions | umls-concept:C0719453 | lld:lifeskim |
| pubmed-article:15734732 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:15734732 | pubmed:dateCreated | 2005-4-25 | lld:pubmed |
| pubmed-article:15734732 | pubmed:abstractText | The antioxidant response element (ARE) and Nrf2 are known to regulate the expression and coordinated induction of genes encoding detoxifying enzymes including NAD(P)H:quinone oxidoreductase1 (NQO1) in response to antioxidants. In this report, we demonstrate that overexpression of the transcription factor Bach1 in Hep-G2 cells negatively regulated NQO1 gene expression and induction in response to antioxidant t-BHQ. Bandshift and supershift assays revealed that Bach1 binds to the ARE as a heterodimer with small Maf proteins but not as a homodimer or heterodimer with Nrf2. The transfection and ChIP assays revealed that Bach1 and Nrf2 competed with each other to regulate ARE-mediated gene expression. Heme, a negative regulator of Bach1 relieved the Bach1 repression of NQO1 gene expression in transfected cells. The transcription of Bach1 and Nrf2 did not change in response to t-BHQ. Immunofluorescence assays and Western blot analysis revealed that both Bach1 and Nrf2 localized in the cytoplasm and nucleus of the untreated cells. The treatment of cells with t-BHQ resulted in the nuclear accumulation of both Bach1 and Nrf2. Interestingly, the t-BHQ-induced nuclear accumulation of Bach1 was significantly delayed over that of Nrf2. These results led to the conclusion that a balance of Nrf2 versus Bach1 inside the nucleus influences up- or down-regulation of ARE-mediated gene expression. The results further suggest that antioxidant-induced delayed accumulation of Bach1 contributes to the down-regulation of ARE-regulated genes, presumably to reduce the antioxidant enzymes to normal levels. | lld:pubmed |
| pubmed-article:15734732 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15734732 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15734732 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15734732 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15734732 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:15734732 | pubmed:author | pubmed-author:BloomDavid... | lld:pubmed |
| pubmed-article:15734732 | pubmed:author | pubmed-author:Dhakshinamoor... | lld:pubmed |
| pubmed-article:15734732 | pubmed:author | pubmed-author:JaiswalAnil... | lld:pubmed |
| pubmed-article:15734732 | pubmed:author | pubmed-author:JainAbhinav... | lld:pubmed |
| pubmed-article:15734732 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15734732 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:15734732 | pubmed:volume | 280 | lld:pubmed |
| pubmed-article:15734732 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15734732 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15734732 | pubmed:pagination | 16891-900 | lld:pubmed |
| pubmed-article:15734732 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:15734732 | pubmed:meshHeading | pubmed-meshheading:15734732... | lld:pubmed |
| pubmed-article:15734732 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15734732 | pubmed:articleTitle | Bach1 competes with Nrf2 leading to negative regulation of the antioxidant response element (ARE)-mediated NAD(P)H:quinone oxidoreductase 1 gene expression and induction in response to antioxidants. | lld:pubmed |
| pubmed-article:15734732 | pubmed:affiliation | Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA. | lld:pubmed |
| pubmed-article:15734732 | pubmed:publicationType | Journal Article | lld:pubmed |
