rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-2-28
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pubmed:abstractText |
PGC-1alpha mRNA and protein are elevated in islets from multiple animal models of diabetes. Overexpression of PGC-1alpha impairs glucose-stimulated insulin secretion (GSIS). However, it is not well known which metabolic events lead to upregulation of PGC-1alpha in the beta-cells under pathophysiological condition. In present study, we have investigated effects of chronic hyperlipidemia and hyperglycemia on PGC-1alpha mRNA expression in isolated rat islets. Isolated rat islets are chronically incubated with 0, 0.2 and 0.4 mM oleic acid/palmitic acid (free fatty acids, FFA) or 5.5 and 25 mM glucose for 72 h. FFA dose-dependently increases PGC-1alpha mRNA expression level in isolated islets. FFA also increases PGC-1alpha expression in mouse beta-cell-derived beta TC3 cell line. In contrast, 25 mM glucose decreases expression level of PGC-1alpha. Inhibition of PGC-1alpha by siRNA improves FFA-induced impairment of GSIS in islets. These data suggest that hyperlipidemia and hyperglycemia regulate PGC-1alpha expression in islets differently, and elevated PGC-1alpha by FFA plays an important role in chronic hyperlipidemia-induced beta-cell dysfunction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Nonesterified,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon-Like Peptide 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/peroxisome-proliferator-activated...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
579
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1446-52
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15733855-Animals,
pubmed-meshheading:15733855-Cells, Cultured,
pubmed-meshheading:15733855-Fatty Acids, Nonesterified,
pubmed-meshheading:15733855-Forskolin,
pubmed-meshheading:15733855-Gene Expression Regulation,
pubmed-meshheading:15733855-Glucagon,
pubmed-meshheading:15733855-Glucagon-Like Peptide 1,
pubmed-meshheading:15733855-Glucose,
pubmed-meshheading:15733855-Islets of Langerhans,
pubmed-meshheading:15733855-Liver,
pubmed-meshheading:15733855-Male,
pubmed-meshheading:15733855-Mice,
pubmed-meshheading:15733855-Peptide Fragments,
pubmed-meshheading:15733855-Protein Precursors,
pubmed-meshheading:15733855-RNA, Messenger,
pubmed-meshheading:15733855-RNA, Small Interfering,
pubmed-meshheading:15733855-Rats,
pubmed-meshheading:15733855-Rats, Wistar,
pubmed-meshheading:15733855-Transcription Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Free fatty acids increase PGC-1alpha expression in isolated rat islets.
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pubmed:affiliation |
Institute of Mecular Medicine and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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