Linked Life Data

15730236

Source:http://linkedlifedata.com/resource/pubmed/id/15730236

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-2-25
pubmed:abstractText
NMR spectroscopy has been coupled on-line to capillary isotachophoresis (cITP) to enhance structural analyses of dilute charged species through separation and sample concentration. Microcoils, the most mass-sensitive NMR probes available, provide optimal detection for cITP/NMR. To evaluate the utility of cITP/NMR for natural product analysis, a homogenate of the hypobranchial gland from the marine snail Calliostoma canaliculatum containing a cationic neurotoxin (1, a disulfide-bonded dimer of 6-bromo-2-mercaptotryptamine) was studied. For comparison, hypobranchial gland homogenate was also examined by diffusion-NMR, an alternative approach for NMR mixture analysis. cITP/NMR concentrated the neurotoxin by almost 20-fold and isolated it from some of the other components present in the matrix. However, a minor component, likely a precursor or degradant, co-migrated with compound 1. Diffusion-NMR also did not resolve the two, indicating that the compounds possessed similar diffusion coefficients and electrophoretic mobilities. The strengths and limitations of the two approaches for NMR mixture analysis are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0163-3864
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
162-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Comparative analysis of a neurotoxin from Calliostoma canaliculatum by on-line capillary isotachophoresis/1H NMR and diffusion 1H NMR.
pubmed:affiliation
Department of Chemistry and the Beckman Institute, University of Illinois, Urbana, IL 61801, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.