| pubmed-article:1572972 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C0027442 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C0162633 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C0086231 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C0450099 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:1572972 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:1572972 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1572972 | pubmed:dateCreated | 1992-5-29 | lld:pubmed |
| pubmed-article:1572972 | pubmed:abstractText | We developed a direct enzyme immunoassay [EIA; Enzygnost Influenza A(Ag) and Enzygnost Influenza B(Ag)] for the direct detection of influenza A and B virus antigens in nasopharyngeal secretion specimens (NPS). The test is performed without sonification of specimens, and results are obtained within 4 h. A direct comparison between direct EIA and quantitation of virus shedding for influenza A and B virus antigen detection was carried out. A total of 210 NPS and 98 nasopharyngeal wash specimens (NPW) were investigated. We isolated influenza A viruses from 79 (37.6%) of 210 NPS; of these 79 cell-culture-positive NPS, 70 (88.6%) were also positive by direct EIA. Of 29 (13.8%) NPS from which influenza B virus was isolated, 24 (82.8%) NPS were positive by direct EIA. Virus shedding was determined quantitatively in 48 NPS from patients with influenza A and in 24 NPS from patients with influenza B. Only a crude correlation between optical density values and virus concentrations was observed. Detection of influenza virus antigens in NPS by direct EIA showed sensitivities of 89.7% for influenza A virus and 87.9% for influenza B virus and specificities of 99.3% for influenza A virus and 100% for influenza B virus. With direct EIA, all NPW were negative for influenza A virus, although virus was isolated from 21 (21.4%) NPW. Of 15 NPW from which influenza B virus was isolated, 7 showed positive results in direct EIA. In addition, direct EIA is suitable for detecting influenza A and B viruses in cell cultures before the appearance of any cytopathic effects and can be used as a cell culture confirmation test. | lld:pubmed |
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| pubmed-article:1572972 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1572972 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1572972 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1572972 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1572972 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1572972 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1572972 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:1572972 | pubmed:author | pubmed-author:GerthH JHJ | lld:pubmed |
| pubmed-article:1572972 | pubmed:author | pubmed-author:TjhenK YKY | lld:pubmed |
| pubmed-article:1572972 | pubmed:author | pubmed-author:DöllerGG | lld:pubmed |
| pubmed-article:1572972 | pubmed:author | pubmed-author:SchuyWW | lld:pubmed |
| pubmed-article:1572972 | pubmed:author | pubmed-author:StekelerBB | lld:pubmed |
| pubmed-article:1572972 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1572972 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:1572972 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1572972 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1572972 | pubmed:pagination | 866-9 | lld:pubmed |
| pubmed-article:1572972 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:1572972 | pubmed:meshHeading | pubmed-meshheading:1572972-... | lld:pubmed |
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| pubmed-article:1572972 | pubmed:meshHeading | pubmed-meshheading:1572972-... | lld:pubmed |
| pubmed-article:1572972 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1572972 | pubmed:articleTitle | Direct detection of influenza virus antigen in nasopharyngeal specimens by direct enzyme immunoassay in comparison with quantitating virus shedding. | lld:pubmed |
| pubmed-article:1572972 | pubmed:affiliation | Department of Medical Virology, Hygiene Institute, Tübingen, Germany. | lld:pubmed |
| pubmed-article:1572972 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1572972 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1572972 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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