|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1992-5-29
|
|
pubmed:abstractText |
We developed a direct enzyme immunoassay [EIA; Enzygnost Influenza A(Ag) and Enzygnost Influenza B(Ag)] for the direct detection of influenza A and B virus antigens in nasopharyngeal secretion specimens (NPS). The test is performed without sonification of specimens, and results are obtained within 4 h. A direct comparison between direct EIA and quantitation of virus shedding for influenza A and B virus antigen detection was carried out. A total of 210 NPS and 98 nasopharyngeal wash specimens (NPW) were investigated. We isolated influenza A viruses from 79 (37.6%) of 210 NPS; of these 79 cell-culture-positive NPS, 70 (88.6%) were also positive by direct EIA. Of 29 (13.8%) NPS from which influenza B virus was isolated, 24 (82.8%) NPS were positive by direct EIA. Virus shedding was determined quantitatively in 48 NPS from patients with influenza A and in 24 NPS from patients with influenza B. Only a crude correlation between optical density values and virus concentrations was observed. Detection of influenza virus antigens in NPS by direct EIA showed sensitivities of 89.7% for influenza A virus and 87.9% for influenza B virus and specificities of 99.3% for influenza A virus and 100% for influenza B virus. With direct EIA, all NPW were negative for influenza A virus, although virus was isolated from 21 (21.4%) NPW. Of 15 NPW from which influenza B virus was isolated, 7 showed positive results in direct EIA. In addition, direct EIA is suitable for detecting influenza A and B viruses in cell cultures before the appearance of any cytopathic effects and can be used as a cell culture confirmation test.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-1975413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2037665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2199488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2203125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2322232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2547019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2643611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2674180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2808674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2851878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-2997270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3024963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3045148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3517049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3533980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3547056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3600670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3742798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-387816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-3902885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-6764777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1572972-7026733
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
30
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
866-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:1572972-Antigens, Viral,
pubmed-meshheading:1572972-Cytopathogenic Effect, Viral,
pubmed-meshheading:1572972-Evaluation Studies as Topic,
pubmed-meshheading:1572972-Humans,
pubmed-meshheading:1572972-Immunoenzyme Techniques,
pubmed-meshheading:1572972-Influenza, Human,
pubmed-meshheading:1572972-Influenza A virus,
pubmed-meshheading:1572972-Influenza B virus,
pubmed-meshheading:1572972-Nasopharynx,
pubmed-meshheading:1572972-Orthomyxoviridae
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Direct detection of influenza virus antigen in nasopharyngeal specimens by direct enzyme immunoassay in comparison with quantitating virus shedding.
|
|
pubmed:affiliation |
Department of Medical Virology, Hygiene Institute, Tübingen, Germany.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
