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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2005-2-24
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pubmed:abstractText |
Experimental studies suggest that the delivery of antiepileptic agents into the seizure focus might be of potential utility for the treatment of focal-onset epilepsies. Botulinum neurotoxin E (BoNT/E) causes a prolonged inhibition of neurotransmitter release after its specific cleavage of the synaptic protein synaptosomal-associated protein of 25 kDa (SNAP-25). Here, we show that BoNT/E injected into the rat hippocampus inhibits glutamate release and blocks spike activity of pyramidal neurons. BoNT/E effects persist for at least 3 weeks, as determined by immunodetection of cleaved SNAP-25 and loss of intact SNAP-25. The delivery of BoNT/E to the rat hippocampus dramatically reduces both focal and generalized kainic acid-induced seizures as documented by behavioral and electrographic analysis. BoNT/E treatment also prevents neuronal loss and long-term cognitive deficits associated with kainic acid seizures. Moreover, BoNT/E-injected rats require 50% more electrical stimulations to reach stage 5 of kindling, thus indicating a delayed epileptogenesis. We conclude that BoNT/E delivery to the hippocampus is both antiictal and antiepileptogenic in experimental models of epilepsy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anticonvulsants,
http://linkedlifedata.com/resource/pubmed/chemical/Botulinum Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Convulsants,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Snap25 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptosomal-Associated Protein 25,
http://linkedlifedata.com/resource/pubmed/chemical/botulinum toxin type E
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1529-2401
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pubmed:author |
pubmed-author:AntonucciFlaviaF,
pubmed-author:BozziYuriY,
pubmed-author:CaleoMatteoM,
pubmed-author:CostantinLauraL,
pubmed-author:FunicelloMarcellaM,
pubmed-author:GobbiMarcoM,
pubmed-author:MaffeiLambertoL,
pubmed-author:MenniniTizianaT,
pubmed-author:MontecuccoCesareC,
pubmed-author:RichichiCristinaC,
pubmed-author:RossettoOrnellaO,
pubmed-author:VezzaniAnnamariaA,
pubmed-author:ViegiAlessandroA
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pubmed:issnType |
Electronic
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pubmed:day |
23
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1943-51
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15728834-Animals,
pubmed-meshheading:15728834-Anticonvulsants,
pubmed-meshheading:15728834-Botulinum Toxins,
pubmed-meshheading:15728834-Cell Death,
pubmed-meshheading:15728834-Cognition Disorders,
pubmed-meshheading:15728834-Convulsants,
pubmed-meshheading:15728834-Drug Evaluation, Preclinical,
pubmed-meshheading:15728834-Electric Stimulation,
pubmed-meshheading:15728834-Electroencephalography,
pubmed-meshheading:15728834-Epilepsies, Partial,
pubmed-meshheading:15728834-Epilepsy, Generalized,
pubmed-meshheading:15728834-Glutamic Acid,
pubmed-meshheading:15728834-Hippocampus,
pubmed-meshheading:15728834-Injections, Intralesional,
pubmed-meshheading:15728834-Kainic Acid,
pubmed-meshheading:15728834-Kindling, Neurologic,
pubmed-meshheading:15728834-Maze Learning,
pubmed-meshheading:15728834-Membrane Proteins,
pubmed-meshheading:15728834-Nerve Tissue Proteins,
pubmed-meshheading:15728834-Pyramidal Cells,
pubmed-meshheading:15728834-Random Allocation,
pubmed-meshheading:15728834-Rats,
pubmed-meshheading:15728834-Rats, Long-Evans,
pubmed-meshheading:15728834-Stereotaxic Techniques,
pubmed-meshheading:15728834-Synaptosomal-Associated Protein 25
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pubmed:year |
2005
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pubmed:articleTitle |
Antiepileptic effects of botulinum neurotoxin E.
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pubmed:affiliation |
Scuola Normale Superiore, Consiglio Nazionale delle Ricerche, 56100 Pisa, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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