Linked Life Data

15726426

Source:http://linkedlifedata.com/resource/pubmed/id/15726426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-2-23
pubmed:abstractText
To ascertain whether the PTEN (phosphatase and tensin homolog deleted on chromosome 10)/Akt signaling pathway is activated during ischemic brain injury, we investigated the expression and phosphorylation of PTEN and Akt by immunohistochemistry in the rat hippocampus after transient forebrain ischemia. Weak immunoreactivity for PTEN and its phosphorylated form (p-PTEN) was constitutively expressed in hippocampal neurons and astrocytes of the control rats, but their upregulation was detected mainly in reactive astrocytes in the ischemic hippocampus. Increased immunoreactivity for PTEN and p-PTEN occurred specifically in astrocytes by day 1 and was sustained for more than 2 weeks. The spatiotemporal activation of Akt in the ischemic hippocampus mirrored that of p-PTEN expression. Post-ischemic activation of Akt, revealed by phosphorylated Akt (p-Akt) immunoreactivity, was first detected at day 1 and was maintained for at least 2 weeks. Double-labeling experiments revealed that the cells expressing PTEN, p-PTEN, or p-Akt were reactive astrocytes expressing glial fibrillary acidic protein. These results demonstrate the increased phosphorylation of PTEN and Akt in reactive astrocytes of the post-ischemic hippocampus, suggesting that the PTEN/Akt pathway is involved in the astroglial reaction in the rat hippocampus after transient forebrain ischemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
319
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-66
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15726426-Animals, pubmed-meshheading:15726426-Astrocytes, pubmed-meshheading:15726426-Biological Markers, pubmed-meshheading:15726426-Fluorescent Antibody Technique, pubmed-meshheading:15726426-Glial Fibrillary Acidic Protein, pubmed-meshheading:15726426-Hippocampus, pubmed-meshheading:15726426-Immunoenzyme Techniques, pubmed-meshheading:15726426-Ischemic Attack, Transient, pubmed-meshheading:15726426-Male, pubmed-meshheading:15726426-PTEN Phosphohydrolase, pubmed-meshheading:15726426-Phosphorylation, pubmed-meshheading:15726426-Protein Tyrosine Phosphatases, pubmed-meshheading:15726426-Protein-Serine-Threonine Kinases, pubmed-meshheading:15726426-Proto-Oncogene Proteins, pubmed-meshheading:15726426-Proto-Oncogene Proteins c-akt, pubmed-meshheading:15726426-Rats, pubmed-meshheading:15726426-Rats, Sprague-Dawley
pubmed:year
2005
pubmed:articleTitle
Phosphorylation of PTEN and Akt in astrocytes of the rat hippocampus following transient forebrain ischemia.
pubmed:affiliation
Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul, 137-701, Korea. munylee@catholic.ac.kr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't