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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0006784,
umls-concept:C0007589,
umls-concept:C0007600,
umls-concept:C0007634,
umls-concept:C0036667,
umls-concept:C0070750,
umls-concept:C0205251,
umls-concept:C0205263,
umls-concept:C0205369,
umls-concept:C0312418,
umls-concept:C0442805,
umls-concept:C0600432,
umls-concept:C1120859,
umls-concept:C1511938
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pubmed:issue |
2
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pubmed:dateCreated |
1992-6-2
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pubmed:abstractText |
Changes in the level of calcium-activated neutral proteases (calpains) in K562 cells induced to differentiate by phorbol 12-myristate 13-acetate (PMA) were examined by an immunohistochemical technique and Western blot analysis. A remarkable increase in m-calpain (high-Ca(2+)-requiring form) level was detected after PMA-treatment, while there was no significant difference in mu-calpain (low-Ca(2+)-requiring form) level between PMA-treated and untreated K562 cells. To confirm whether the increase in m-calpain is specific to PMA-induced differentiation, we examined changes in calpain in K562 cells cultured in serum-free medium and in synchronized cells. The results indicate that the increase has no relation to growth arrest or to cell cycle. PMA-treated cells exhibited increased nonspecific esterase activity, suggesting monocytic differentiation. Immunoelectron microscopic study showed the reactions of dense deposits with monoclonal anti-m-calpain antibody on cell membranes, on membranes of coated vesicles, and on rough endoplasmic reticulum of K562 cells after 26 h of PMA treatment.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-4827
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
200
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
513-22
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1572412-Blotting, Western,
pubmed-meshheading:1572412-Calpain,
pubmed-meshheading:1572412-Cell Differentiation,
pubmed-meshheading:1572412-Cell Nucleus,
pubmed-meshheading:1572412-Enzyme Activation,
pubmed-meshheading:1572412-Erythroblasts,
pubmed-meshheading:1572412-Humans,
pubmed-meshheading:1572412-Immunoenzyme Techniques,
pubmed-meshheading:1572412-Leukemia, Myeloid, Chronic-Phase,
pubmed-meshheading:1572412-Microscopy, Electron,
pubmed-meshheading:1572412-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1572412-Time Factors,
pubmed-meshheading:1572412-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Specific increase in calcium-activated neutral protease with low calcium sensitivity (m-calpain) in proerythroblastic K562 cell line cells induced to differentiation by phorbol 12-myristate 13-acetate.
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pubmed:affiliation |
Department of Enzyme Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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