Linked Life Data

15718027

Source:http://linkedlifedata.com/resource/pubmed/id/15718027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-2-18
pubmed:abstractText
Plasmid pEF1071 (9328 bp), bearing the structural genes of enterocins 1071A and 1071B, had been sequenced and nine genes were identified. The genes responsible for the production and transfer of enterocins 1071A and 1071B are arranged in two operons. The first operon (EntABI) contains the genes ent1071A, ent1071B and entI that encodes enterocin 1071A, enterocin 1071B and immunity to these bacteriocins, respectively. The second operon (EntTD) contains two genes, viz. abc (also named entT) and entD, encoding a putative ABC transporter (697 aa) and an accessory protein (697 aa), respectively. Three genes (mobC, mobA and mobX), situated downstream of the second operon, encode proteins of 127, 346 and 224 amino acids, respectively, and are presumably involved in the mobilization of plasmid pEF1071. The ninth gene was identified as a putative repA gene encoding a protein of 327 amino acids. The transcription initiation sites of the genes ent1071A, abc, mobA, mobX and repA were determined by primer extension. By inserting the cat gene into the ent1071B gene, and thereby disrupting the operon structure, we have shown that bacteriocins Ent1071A and Ent1071B act independently against target bacteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0168-1605
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-45
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Molecular analysis of the gene cluster involved in the production and secretion of enterocins 1071A and 1071B and of the genes responsible for the replication and transfer of plasmid pEF1071.
pubmed:affiliation
Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa.
pubmed:publicationType
Journal Article