Linked Life Data

15715966

Source:http://linkedlifedata.com/resource/pubmed/id/15715966

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-4-12
pubmed:abstractText
Mammalian Trio is a multifunctional, multidomain Rho guanine nucleotide exchange factor (GEF) closely related to Kalirin. Trio is important for proper axon guidance in Drosophila, and mice lacking Trio exhibit both skeletal muscle and neuronal disorders. Full length mammalian Trio and Kalirin both consist of a Sec14P-like domain, several spectrin-like domains, two Rho GEF domains each containing a Dbl-homology (DH) and a pleckstrin-homology (PH) domain, two src homology 3 domains (SH3), Ig/fibronectin-like domains (Ig/FN), and a kinase domain. We have previously described multiple isoforms of Kalirin derived through alternative splicing and multiple transcription start sites, but multiple isoforms of Trio containing different functional domains have not been described. Using a new antibody directed against the spectrin-like region of rat Trio coupled with reverse transcription PCR and cDNA sequencing, we have identified 4 novel isoforms of Trio expressed in rat cortex and cerebellum. Two isoforms, Trio 9S and Trio 9L, are derived through alternative splicing of Trio exon 48 and are abundantly expressed in rat brain. Trio 8 is expressed in postnatal day 30 and adult cerebellum, but not in cortex or skeletal muscle. Trio/duet is expressed in adult cortex and cerebellum. In the rat brain, each of these Trio isoforms is expressed at a higher level than full length Trio.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
347
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-35
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15715966-Alternative Splicing, pubmed-meshheading:15715966-Amino Acid Sequence, pubmed-meshheading:15715966-Animals, pubmed-meshheading:15715966-Cerebellum, pubmed-meshheading:15715966-DNA, Complementary, pubmed-meshheading:15715966-Gene Expression Regulation, pubmed-meshheading:15715966-Guanine Nucleotide Exchange Factors, pubmed-meshheading:15715966-Humans, pubmed-meshheading:15715966-Molecular Sequence Data, pubmed-meshheading:15715966-Nerve Tissue Proteins, pubmed-meshheading:15715966-Phosphoproteins, pubmed-meshheading:15715966-Protein Isoforms, pubmed-meshheading:15715966-Protein Structure, Tertiary, pubmed-meshheading:15715966-Rats, pubmed-meshheading:15715966-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15715966-Sequence Homology, Amino Acid, pubmed-meshheading:15715966-Spectrin
pubmed:year
2005
pubmed:articleTitle
Multiple novel isoforms of Trio are expressed in the developing rat brain.
pubmed:affiliation
Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3401, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural