Linked Life Data

15714585

Source:http://linkedlifedata.com/resource/pubmed/id/15714585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-3-2
pubmed:abstractText
Using adoptive transfer of TCR-transgenic T cells, we examined the homing of transgenic T cells to splenic compartments in situ. After systemic immunization with peptide or protein antigen, the location of clonotypic T cells, cytokine production, cell surface markers, and apoptosis were assessed. There were distinct differences in the splenic homing of CD4(+) TCR-transgenic T cells in mice immunized with peptide as compared to mice immunized with whole-protein antigen. T cells in peptide-immunized mice were found almost exclusively in the splenic red pulp, but not in the T and B cell zones (white pulp), while the majority of T cells immunized with whole protein were found in the white pulp. Many more Fas ligand-expressing and apoptotic cells were present after peptide immunization than after whole-protein immunization. Localization of IL-4-, IL-2- and IFN-gamma-producing cells to the lymphocyte-containing splenic white pulp was only observed with whole-protein immunization. The unique homing and increased apoptosis of immune cells post peptide immunization may help explain the ineffectiveness of many peptide vaccines. Linkage of the same peptide epitope to a carrier protein increased white pulp T cell localization and decreased apoptosis, suggesting a strategy to enhance peptide vaccine responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
776-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15714585-Adoptive Transfer, pubmed-meshheading:15714585-Animals, pubmed-meshheading:15714585-Apoptosis, pubmed-meshheading:15714585-CD40 Ligand, pubmed-meshheading:15714585-Carrier Proteins, pubmed-meshheading:15714585-Cell Movement, pubmed-meshheading:15714585-Cytokines, pubmed-meshheading:15714585-Fas Ligand Protein, pubmed-meshheading:15714585-Immunization, pubmed-meshheading:15714585-Immunohistochemistry, pubmed-meshheading:15714585-In Situ Nick-End Labeling, pubmed-meshheading:15714585-Membrane Glycoproteins, pubmed-meshheading:15714585-Mice, pubmed-meshheading:15714585-Mice, Transgenic, pubmed-meshheading:15714585-Ovalbumin, pubmed-meshheading:15714585-Peptide Fragments, pubmed-meshheading:15714585-Receptors, Antigen, T-Cell, pubmed-meshheading:15714585-Spleen, pubmed-meshheading:15714585-T-Lymphocytes
pubmed:year
2005
pubmed:articleTitle
Peptide immunization excludes antigen-specific T cells from splenic lymphoid compartments.
pubmed:affiliation
Division of Monoclonal Antibodies, Office of Biotechnology Products, Office of Pharmaceutical Sciences, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, USA.
pubmed:publicationType
Journal Article, Comparative Study