Linked Life Data

15712879

Source:http://linkedlifedata.com/resource/pubmed/id/15712879

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-2-16
pubmed:abstractText
2-(9-Carbazole)-ethyl chloroformate (CEOC) has been used for the pre-column derivatization of amino acids in micellar electrokinetic capillary chromatography (MEKC). Nine amino acid standards (Ser, Thr, Gly, Glu, Asp, Val, Ile, Leu, Phe) were labeled with CEOC and separated in a buffer system containing 20 mmol/L borate-30 mmol/L sodium lauryl sulfate (SDS) and 3% acetonitrile at pH 9.0 with an applied voltage of 18 kV and column temperature of 25 degrees C. The derivatives were detected at UV 214 nm. Under optimal conditions, this method offers a baseline separation for nine CEOC-derivatized amino acids within 14 min. The linearity range was from 0.025 to 0.25 mmol/L and the detection limits were from 2.15 to 2.46 micromol/L.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1000-8713
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
166-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
[Micellar electrokinetic capillary chromatographic separation of amino acids derivatized with 2-(9-carbazole)-ethyl chloroformate as a pre-column labeling reagent].
pubmed:affiliation
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't