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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1992-5-29
|
| pubmed:abstractText |
Fluorescent in situ hybridization has become a major technique for visualizing genetic material in fixed cells. Currently, many systems utilize the hybridization of labeled molecular probes to cells that are attached to slides. We have developed a technique that allows for in situ hybridization to be performed using cells in suspension. By using digoxigenin-labeled DNA probes and a fluoresceinated antibody directed against the digoxigenin, we can measure the resulting signal on a flow cytometer and the cells can be attached to microscope slides for visual analysis.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
362-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1571143-Animals,
pubmed-meshheading:1571143-Base Sequence,
pubmed-meshheading:1571143-Biotechnology,
pubmed-meshheading:1571143-DNA,
pubmed-meshheading:1571143-Digoxigenin,
pubmed-meshheading:1571143-Flow Cytometry,
pubmed-meshheading:1571143-Fluorescent Antibody Technique,
pubmed-meshheading:1571143-Molecular Probes,
pubmed-meshheading:1571143-Molecular Sequence Data,
pubmed-meshheading:1571143-Nucleic Acid Hybridization,
pubmed-meshheading:1571143-RNA, Messenger
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Fluorescent in situ hybridization en suspension (FISHES) using digoxigenin-labeled probes and flow cytometry.
|
| pubmed:affiliation |
Department of Flow Cytometry, Roswell Park Cancer Institute, Buffalo, NY 14263.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|