rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1992-6-4
|
pubmed:abstractText |
The alpha 1 alpha 2 alpha 3-chain form of human type V collagen was solubilized from placenta by pepsin treatment and isolated by ion-exchange chromatography. The alpha 3-chain was further separated after denaturation of the triple helix also by ion-exchange chromatography, cleaved with lysyl endopeptidase and the fragments separated by size-exclusion chromatography and reversed phase HPLC. N-Terminal sequence analysis of the fragments and comparison to sequences contained in a database indicated a relatively high similarity of the alpha 3(V)-chain to alpha 1(V) and alpha 1(XI) with an identity of approximately 73%.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0177-3593
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
373
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
69-75
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1571108-Amino Acid Sequence,
pubmed-meshheading:1571108-Animals,
pubmed-meshheading:1571108-Chromatography, Gel,
pubmed-meshheading:1571108-Chromatography, High Pressure Liquid,
pubmed-meshheading:1571108-Chromatography, Ion Exchange,
pubmed-meshheading:1571108-Collagen,
pubmed-meshheading:1571108-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1571108-Female,
pubmed-meshheading:1571108-Humans,
pubmed-meshheading:1571108-Macromolecular Substances,
pubmed-meshheading:1571108-Molecular Sequence Data,
pubmed-meshheading:1571108-Pepsin A,
pubmed-meshheading:1571108-Peptide Fragments,
pubmed-meshheading:1571108-Placenta,
pubmed-meshheading:1571108-Pregnancy,
pubmed-meshheading:1571108-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1571108-Vertebrates
|
pubmed:year |
1992
|
pubmed:articleTitle |
Isolation of the alpha 3-chain of human type V collagen and characterization by partial sequencing.
|
pubmed:affiliation |
Max-Planck-Institut für Biochemie, Martinsried, Germany.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|