Source:http://linkedlifedata.com/resource/pubmed/id/15710393
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2005-2-15
|
| pubmed:abstractText |
Import of tRNAs into plant mitochondria appears to be highly specific. We recently showed that the anticodon and the D-domain sequences are essential determinants for tRNAVal import into tobacco cell mitochondria. To determine the minimal set of elements required to direct import of a cytosol-specific tRNA species, tobacco cells were transformed with an Arabidopsis thaliana intron-containing tRNAMet-e gene carrying the D-domain and the anticodon of a valine tRNA. Although well expressed and processed into tobacco cells, this mutated tRNA was shown to remain in the cytosol. Furthermore, a mutant tRNAVal carrying the T-domain of the tRNAMet-e, although still efficiently recognized by the valyl-tRNA synthetase, is not imported into mitochondria. Altogether these results suggest that mutations affecting the core of a tRNA molecule also alter its import ability into plant mitochondria.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0014-5793
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
14
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| pubmed:volume |
579
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1072-8
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15710393-Arabidopsis,
pubmed-meshheading:15710393-Base Sequence,
pubmed-meshheading:15710393-Cell Line,
pubmed-meshheading:15710393-Gene Expression Regulation, Plant,
pubmed-meshheading:15710393-Introns,
pubmed-meshheading:15710393-Kinetics,
pubmed-meshheading:15710393-Mitochondria,
pubmed-meshheading:15710393-Molecular Sequence Data,
pubmed-meshheading:15710393-Mutation,
pubmed-meshheading:15710393-Nucleic Acid Conformation,
pubmed-meshheading:15710393-Plants, Genetically Modified,
pubmed-meshheading:15710393-RNA, Transfer, Amino Acyl,
pubmed-meshheading:15710393-RNA, Transfer, Met,
pubmed-meshheading:15710393-RNA Splice Sites,
pubmed-meshheading:15710393-RNA Transport,
pubmed-meshheading:15710393-Tobacco,
pubmed-meshheading:15710393-Transcription, Genetic,
pubmed-meshheading:15710393-Transfer RNA Aminoacylation
|
| pubmed:year |
2005
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| pubmed:articleTitle |
The T-domain of cytosolic tRNAVal, an essential determinant for mitochondrial import.
|
| pubmed:affiliation |
Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|