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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-2-15
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pubmed:abstractText |
Haem controls its own synthesis in non-erythroid cells primarily by regulation of ALAS1 mRNA stability. Alternative splicing of human ALAS1 generates two mRNAs with different 5'-UTRs: a major one, where exon 1B is omitted, and a minor form containing exon 1B. We show that, unlike the major ALAS1 mRNA, the minor form was resistant to haem-mediated decay. Furthermore, we demonstrate that the ALAS1 5'-UTR alone did not confer haem-mediated decay upon a heterologous mRNA and the inclusion of exon 1B inhibited translation. These data suggest that translation of ALAS1 mRNA itself might be required for destabilisation in response to haem.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
579
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1061-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15710391-5' Untranslated Regions,
pubmed-meshheading:15710391-5-Aminolevulinate Synthetase,
pubmed-meshheading:15710391-Alternative Splicing,
pubmed-meshheading:15710391-Cell Line, Tumor,
pubmed-meshheading:15710391-Dactinomycin,
pubmed-meshheading:15710391-Exons,
pubmed-meshheading:15710391-Heme,
pubmed-meshheading:15710391-Humans,
pubmed-meshheading:15710391-Protein Biosynthesis,
pubmed-meshheading:15710391-RNA, Messenger,
pubmed-meshheading:15710391-RNA Stability
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pubmed:year |
2005
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|
pubmed:articleTitle |
An alternatively-spliced exon in the 5'-UTR of human ALAS1 mRNA inhibits translation and renders it resistant to haem-mediated decay.
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pubmed:affiliation |
Department of Medical Biochemistry and Immunology, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, Wales, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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