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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-2-14
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pubmed:abstractText |
p53 is a tumor suppressor gene whose regulation is crucial to maintaining genome stability and for the apoptotic elimination of abnormal, potentially cancer-predisposing cells. C. elegans contains a primordial p53 gene, cep-1, that acts as a transcription factor necessary for DNA damage-induced apoptosis. In a genetic screen for negative regulators of CEP-1, we identified a mutation in GLD-1, a translational repressor implicated in multiple C. elegans germ cell fate decisions and related to mammalian Quaking proteins. CEP-1-dependent transcription of proapoptotic genes is upregulated in the gld-1(op236) mutant and an elevation of p53-mediated germ cell apoptosis in response to DNA damage is observed. Further, we demonstrate that GLD-1 mediates its repressive effect by directly binding to the 3'UTR of cep-1/p53 mRNA and repressing its translation. This study reveals that the regulation of cep-1/p53 translation influences DNA damage-induced apoptosis and demonstrates the physiological importance of this mechanism.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
357-68
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15707894-3' Untranslated Regions,
pubmed-meshheading:15707894-Animals,
pubmed-meshheading:15707894-Apoptosis,
pubmed-meshheading:15707894-Binding Sites,
pubmed-meshheading:15707894-Caenorhabditis elegans,
pubmed-meshheading:15707894-Caenorhabditis elegans Proteins,
pubmed-meshheading:15707894-DNA Damage,
pubmed-meshheading:15707894-Female,
pubmed-meshheading:15707894-Gene Expression Regulation,
pubmed-meshheading:15707894-Germ Cells,
pubmed-meshheading:15707894-Male,
pubmed-meshheading:15707894-Mutation,
pubmed-meshheading:15707894-RNA, Messenger,
pubmed-meshheading:15707894-Repressor Proteins,
pubmed-meshheading:15707894-Sex Differentiation,
pubmed-meshheading:15707894-Tumor Suppressor Protein p53,
pubmed-meshheading:15707894-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
Translational repression of C. elegans p53 by GLD-1 regulates DNA damage-induced apoptosis.
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pubmed:affiliation |
Department of Cell Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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