Linked Life Data

1570680

Source:http://linkedlifedata.com/resource/pubmed/id/1570680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1992-5-28
pubmed:abstractText
A simple and reliable procedure is described for isolating the cold agglutinins associated with Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque, resuspended in RPMI-medium and warmed to 40 degrees C. The cold agglutinins could be removed from the supernatant by a subsequent centrifugation. Double immunodiffusion, polyacrylamide gel electrophoresis and immunoblotting showed that the cold agglutinins isolated consisted of IgM antibodies exclusively. Their ability to induce agglutination in cooled erythrocytes from healthy pigs confirmed that they were genuine cold agglutinins. The method paves the way for more detailed investigation into the mechanisms of this agglutinating disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0165-2427
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-201
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Isolation of cold agglutinins in Eperythrozoon suis-infected pigs.
pubmed:affiliation
Department of General Pathology and Neuropathology, University of Munich, Germany.
pubmed:publicationType
Journal Article