Linked Life Data

15705753

Source:http://linkedlifedata.com/resource/pubmed/id/15705753

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-2-11
pubmed:databankReference
pubmed:abstractText
Adiponectin is an adipocyte-derived hormone that plays an important role in lipid metabolism and glucose homeostasis. Objectives of this study were 1) to determine the presence and distribution of adiponectin and its receptors 1 and 2 (adipoR1 and adipoR2) in porcine tissues; 2) to characterize pig adiponectin, adipoR1, and adipoR2 mRNA levels in various fat depots from three different breeds of pigs; and 3) to study, in stromal-vascular cell culture, the effects of leptin and tumor necrosis factor-alpha (TNFalpha) on pig adiponectin, adipoR1, and adipoR2 gene expression. To this end, fat Chinese Upton Meishan (UM, n = 10), lean Ham Line (HL, n = 10), and Large White (LW, n = 10) gilts were used. We report the isolation of partial cDNA sequences of pig adipoR1 and adipoR2. Porcine-deduced AA sequences share 97 to 100% homology with human and murine sequences. Pig adipoR1 mRNA is abundant in skeletal muscle, visceral fat, and s.c. fat tissues, whereas adipoR2 mRNA is predominantly expressed in liver, heart, skeletal muscle, and visceral and s.c. fat tissues. Pig adiponectin mRNA levels in s.c. and visceral fat tissues were not associated with plasma insulin and glucose in fasting animals. Subcutaneous (r = -0.44, P < 0.05), visceral (r = -0.43, P < 0.05), and total body fat (r = -0.42, P < 0.05) weights were negatively correlated with adiponectin mRNA levels measured in visceral, but not s.c., fat. Pig adipoR1 and adipoR2 mRNA levels, in visceral fat, were less expressed in fat UM gilts than in the lean HL gilts (P < 0.05). Inverse associations were found between s.c. (r = -0.57, P < 0.01), visceral (r = -0.46, P < 0.05), and total body fat (r = -0.56, P < 0.01) weights and adipoR2 mRNA levels in visceral fat only. We were unable to find such associations for adipoR1 mRNA levels in the overall gilt population. The current study demonstrated that TNFalpha downregulates adiponectin and adipoR2, but not adi-poR1, mRNA levels in stromal-vascular cell culture. Moreover, leptin significantly decreased adiponectin mRNA levels, whereas there was no effect on adiponectin receptors. We conclude that adiponectin and adi-poR2 mRNA levels, but not adipoR1, are modulated in pig visceral fat tissues. Furthermore, our results indicate that TNFalpha interferes with adiponectin function by downregulation of adipoR2 but not of adipoR1 mRNA levels in pigs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1525-3163
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
565-78
pubmed:dateRevised
2008-5-15
pubmed:meshHeading
pubmed-meshheading:15705753-Adiponectin, pubmed-meshheading:15705753-Adipose Tissue, pubmed-meshheading:15705753-Amino Acid Sequence, pubmed-meshheading:15705753-Animals, pubmed-meshheading:15705753-Blood Chemical Analysis, pubmed-meshheading:15705753-Body Weight, pubmed-meshheading:15705753-Cells, Cultured, pubmed-meshheading:15705753-DNA Primers, pubmed-meshheading:15705753-Down-Regulation, pubmed-meshheading:15705753-Fatty Acid-Binding Proteins, pubmed-meshheading:15705753-Female, pubmed-meshheading:15705753-Gene Expression, pubmed-meshheading:15705753-Humans, pubmed-meshheading:15705753-Leptin, pubmed-meshheading:15705753-Molecular Sequence Data, pubmed-meshheading:15705753-Receptors, Adiponectin, pubmed-meshheading:15705753-Swine, pubmed-meshheading:15705753-Tumor Necrosis Factor-alpha
pubmed:year
2005
pubmed:articleTitle
Expression of adiponectin and its receptors in swine.
pubmed:affiliation
Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 7C6.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't