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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-5-28
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pubmed:databankReference |
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pubmed:abstractText |
We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a probable K+ channel. KAT1 was cloned by its ability to suppress a K+ transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This suppression is sensitive to known K+ channel blockers, including tetraethylammonium and Ba2+ ions. The KAT1 cDNA contains an open reading frame capable of encoding a 78-kDa protein that shares structural features found in the Shaker superfamily of K+ channels. These include a cluster of six putative membrane-spanning helices (S1-S6) at the amino terminus of the protein, a presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our results suggest that the structural motif for K+ channels has been conserved between plants and animals.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-16667133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-1840699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-1848010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-1899917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2000494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2005786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2022322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2072919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2122519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2122520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2218530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2305265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2415395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2417247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2426783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2441471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2448635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2451788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2463091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2475930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2493160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2659436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-2770868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-3014042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-3037387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-3043197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-5112659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-6033586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-6167991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-6209577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570292-6312838
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:geneSymbol |
KAT1,
ShA,
drk1,
mbk3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3736-40
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1570292-Amino Acid Sequence,
pubmed-meshheading:1570292-Barium,
pubmed-meshheading:1570292-Base Sequence,
pubmed-meshheading:1570292-Cloning, Molecular,
pubmed-meshheading:1570292-DNA,
pubmed-meshheading:1570292-Genes, Plant,
pubmed-meshheading:1570292-Genetic Complementation Test,
pubmed-meshheading:1570292-Membrane Glycoproteins,
pubmed-meshheading:1570292-Molecular Sequence Data,
pubmed-meshheading:1570292-Multigene Family,
pubmed-meshheading:1570292-Plants,
pubmed-meshheading:1570292-Potassium Channels,
pubmed-meshheading:1570292-Saccharomyces cerevisiae,
pubmed-meshheading:1570292-Sequence Alignment,
pubmed-meshheading:1570292-Solubility,
pubmed-meshheading:1570292-Structure-Activity Relationship,
pubmed-meshheading:1570292-Tetraethylammonium Compounds
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pubmed:year |
1992
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pubmed:articleTitle |
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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