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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-2-24
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pubmed:abstractText |
Interferon-alpha (IFN-alpha) is a multifunctional cytokine that modulates immune response. In spite of the numerous comprehensive studies on the effects of IFN-alpha on various cell types, novel characteristics of this versatile agent emerge continuously. In the present study a differential proteomic approach was used to identify new IFN-alpha-regulated proteins in human primary CD4(+) T cells. Two IFN-alpha-inducible proteins, soluble N-ethylmaleimide-sensitive factor attachment protein alpha (alpha-SNAP) and cleavage stimulation factor-64 (CstF-64) previously not described in this context, were identified. Additionally, several proteins already known as IFN-stimulated genes were observed. The results of proteomics experiments were further studied at the mRNA level using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Both peripheral blood and cord blood CD4(+) T cells were used in order to see if there are differences in IFN-alpha response between these populations. Differences were observed between the IFN-alpha-induced expression kinetics in peripheral blood and cord blood transcripts. The induction was more rapid in peripheral blood than in cord blood cells. CstF-64 expression was upregulated by IFN-alpha at the protein, but not at the mRNA level.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1615-9853
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
371-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15700245-Autoradiography,
pubmed-meshheading:15700245-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15700245-Cells, Cultured,
pubmed-meshheading:15700245-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:15700245-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15700245-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15700245-Fetal Blood,
pubmed-meshheading:15700245-Humans,
pubmed-meshheading:15700245-Infant, Newborn,
pubmed-meshheading:15700245-Interferon-alpha,
pubmed-meshheading:15700245-Interferon-gamma,
pubmed-meshheading:15700245-Kinetics,
pubmed-meshheading:15700245-Leukocytes, Mononuclear,
pubmed-meshheading:15700245-Lymphocyte Activation,
pubmed-meshheading:15700245-Peptide Mapping,
pubmed-meshheading:15700245-Proteomics,
pubmed-meshheading:15700245-RNA, Messenger,
pubmed-meshheading:15700245-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15700245-Silver Staining,
pubmed-meshheading:15700245-Solubility,
pubmed-meshheading:15700245-Soluble N-Ethylmaleimide-Sensitive Factor Attachment...,
pubmed-meshheading:15700245-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:15700245-Transcription, Genetic,
pubmed-meshheading:15700245-Vesicular Transport Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
Proteomic and transcriptomic characterization of interferon-alpha-induced human primary T helper cells.
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pubmed:affiliation |
Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Turku, Finland. arsi.rosengren@btk.utu.fi
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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