Source:http://linkedlifedata.com/resource/pubmed/id/15698934
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2005-2-8
|
| pubmed:abstractText |
Rotenone, an inhibitor of mitochondrial respiratory chain complex I, is a useful tool to elicit animal model of Parkinson's disease. Rotenone-induced neuronal apoptosis may contribute to the etiology of Parkinson's disease. However, the mechanism of rotenone-induced apoptosis is not fully understood. In the present study, we show that Ca2+ signaling is essential for rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells. By using Fluo-3/AM and Fura-2/AM, the fluorescent calcium indicator, rotenone was found to cause a rise in intracellular free Ca2+ ([Ca2+]i). The intracellular Ca2+ chelator BAPTA attenuated rotenone-induced apoptosis. Notably, Ca2+ suppression also prevented rotenone-induced apoptotic related events including reactive oxygen species production, G2/M cell cycle arrest and caspase activation, suggesting that Ca2+ signaling is upstream to these events. In the absence of extracellular Ca2+, the rotenone-induced [Ca2+]i elevation was inhibited. Further, the voltage-dependent Ca2+ channel blocker nifedipine suppressed most of the elevation of [Ca2+]i induced by rotenone. These results demonstrate that rotenone leads to an elevation in [Ca2+]i through Ca2+ influx by the opening of voltage-gated Ca2+ channel. This study of rotenone may help to elucidate the neurodegenerative mechanims in Parkinson's disease.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-bis(2-aminophenoxy)ethane-N,N,N'...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Rotenone,
http://linkedlifedata.com/resource/pubmed/chemical/Uncoupling Agents
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0304-3940
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
11
|
| pubmed:volume |
376
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
127-32
|
| pubmed:meshHeading |
pubmed-meshheading:15698934-Apoptosis,
pubmed-meshheading:15698934-Calcium,
pubmed-meshheading:15698934-Calcium Channel Blockers,
pubmed-meshheading:15698934-Caspases,
pubmed-meshheading:15698934-Cell Cycle,
pubmed-meshheading:15698934-Cell Line, Tumor,
pubmed-meshheading:15698934-Chelating Agents,
pubmed-meshheading:15698934-Egtazic Acid,
pubmed-meshheading:15698934-Flow Cytometry,
pubmed-meshheading:15698934-Humans,
pubmed-meshheading:15698934-Intracellular Fluid,
pubmed-meshheading:15698934-Neuroblastoma,
pubmed-meshheading:15698934-Nifedipine,
pubmed-meshheading:15698934-Parkinson Disease,
pubmed-meshheading:15698934-Reactive Oxygen Species,
pubmed-meshheading:15698934-Rotenone,
pubmed-meshheading:15698934-Signal Transduction,
pubmed-meshheading:15698934-Uncoupling Agents
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Possible involvement of Ca2+ signaling in rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells.
|
| pubmed:affiliation |
Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
|
| pubmed:publicationType |
Journal Article
|