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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1992-5-28
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pubmed:abstractText |
Methylation interference and missing contact analyses demonstrate that nuclear factor I (NF I) recognizes an NF I-like site (5'-GGG(N)6GCCAG-3') within the alpha-globin promoter rather than the adjacent CCAAT box. Consistent with this, mutations within the CCAAT box do not alter significantly the affinity and specificity of the interaction whereas elimination of the 5'-GGG-3' half-site of the recognition sequence reduces the DNA binding strength of NF I by 2 orders of magnitude down to the range of unspecific interaction. On the other hand, the mutated alpha-globin promoter sequence that is no longer bound by NF I, although it retains an intact CCAAT box, interacts specifically with a protein component from nuclear extracts of HeLa cells. From these results we conclude that NF I is not the factor that interacts with the CCAAT box and that the second half of the canonical 5'-TGG(N)6GCCAA-3' NF I binding site cannot be regarded as identical with the CCAAT promoter element, as suggested previously.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8478-84
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1569098-Autoradiography,
pubmed-meshheading:1569098-Base Sequence,
pubmed-meshheading:1569098-Binding Sites,
pubmed-meshheading:1569098-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:1569098-DNA Fingerprinting,
pubmed-meshheading:1569098-DNA-Binding Proteins,
pubmed-meshheading:1569098-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1569098-Globins,
pubmed-meshheading:1569098-HeLa Cells,
pubmed-meshheading:1569098-Humans,
pubmed-meshheading:1569098-Methylation,
pubmed-meshheading:1569098-Molecular Sequence Data,
pubmed-meshheading:1569098-NFI Transcription Factors,
pubmed-meshheading:1569098-Nuclear Proteins,
pubmed-meshheading:1569098-Promoter Regions, Genetic,
pubmed-meshheading:1569098-Transcription Factors,
pubmed-meshheading:1569098-Y-Box-Binding Protein 1
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pubmed:year |
1992
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pubmed:articleTitle |
Nuclear factor I (NF I) binds to an NF I-type site but not to the CCAAT site in the human alpha-globin gene promoter.
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pubmed:affiliation |
Institut für Biochemie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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