Source:http://linkedlifedata.com/resource/pubmed/id/15690984
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7-8
|
| pubmed:dateCreated |
2005-2-4
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| pubmed:abstractText |
The transplantation of muscle precursor cells (myoblasts) is a potential therapy for Duchenne muscular dystrophy. A commonly used method to detect cell survival is quantitation of the Y chromosome following transplantation of male donor cells into female hosts. This article presents a direct comparison between real-time quantitative PCR (Q-PCR) and the DNA hybridization (slot-blot) technique for quantitation of Y chromosome DNA. Q-PCR has a significantly greater linear quantitation range and is up to 40-fold more sensitive at low concentrations of male DNA, detecting as little as 1 ng of male DNA in each female tibialis anterior (TA) muscle. At high male DNA concentrations, accurate quantitation by Q-PCR is 2.5 times higher than the maximum possible with slot-blot. In conclusion, Q-PCR has a higher dynamic range and is more efficient than slot-blot analysis for the detection of donor cell engraftment in a transsexual transplantation model.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0963-6897
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
817-21
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15690984-Animals,
pubmed-meshheading:15690984-Cell Survival,
pubmed-meshheading:15690984-Cell Transplantation,
pubmed-meshheading:15690984-Computer Systems,
pubmed-meshheading:15690984-DNA,
pubmed-meshheading:15690984-Disease Models, Animal,
pubmed-meshheading:15690984-Female,
pubmed-meshheading:15690984-Graft Survival,
pubmed-meshheading:15690984-Male,
pubmed-meshheading:15690984-Mice,
pubmed-meshheading:15690984-Mice, Inbred C57BL,
pubmed-meshheading:15690984-Mice, Inbred mdx,
pubmed-meshheading:15690984-Muscle, Skeletal,
pubmed-meshheading:15690984-Muscular Dystrophy, Animal,
pubmed-meshheading:15690984-Myoblasts,
pubmed-meshheading:15690984-Nucleic Acid Hybridization,
pubmed-meshheading:15690984-Polymerase Chain Reaction,
pubmed-meshheading:15690984-Predictive Value of Tests,
pubmed-meshheading:15690984-Sensitivity and Specificity,
pubmed-meshheading:15690984-Y Chromosome
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
A comparison between real-time quantitative PCR and DNA hybridization for quantitation of male DNA following myoblast transplantation.
|
| pubmed:affiliation |
Discipline of Microbiology, School of Biomedical and Chemical Sciences, The University of Western Australia, Perth, WA, Australia.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|