Source:http://linkedlifedata.com/resource/pubmed/id/15689381
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2005-2-11
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| pubmed:abstractText |
Early growth and differentiation of the pancreatic endoderm is regulated by soluble factors from the pancreatic mesenchyme. Previously, we demonstrated that N-cadherin-deficient mice lack a dorsal pancreas, due to a critical role of N-cadherin in dorsal pancreatic mesenchymal cell survival. Here, we show that restoring cardiac and circulatory function in N-cadherin null mice by cardiac-specific expression of N-cadherin, rescues formation of the dorsal pancreas, indicating that the phenotype is secondary to defects related to cardiac/vascular function. Based on this observation, we demonstrate that soluble factors present in plasma, such as sphingosine-1-phosphate, rescue formation of the dorsal pancreas in N-cadherin-deficient mice. We also show that sphingosine-1-phosphate indirectly promotes budding of the pancreatic endoderm by stimulating pancreatic mesenchymal cell proliferation. Finally, we identify sphingosine-1-phosphate receptors within the mesenchyme and show that pertussis toxin blocks the sphingosine-1-phosphate-induced actions, suggesting the involvement of G-protein-coupled sphingosine-1-phosphate receptors. Thus, we propose a new model where blood vessel-derived sphingosine-1-phosphate stimulates growth and budding of the dorsal pancreatic endoderm by induction of mesenchymal cell proliferation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Sepharose,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingosine,
http://linkedlifedata.com/resource/pubmed/chemical/sphingosine 1-phosphate
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0950-1991
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
132
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1085-92
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15689381-Animals,
pubmed-meshheading:15689381-Cadherins,
pubmed-meshheading:15689381-Cell Proliferation,
pubmed-meshheading:15689381-Endoderm,
pubmed-meshheading:15689381-Gene Expression Regulation, Developmental,
pubmed-meshheading:15689381-Genotype,
pubmed-meshheading:15689381-Immunohistochemistry,
pubmed-meshheading:15689381-In Situ Hybridization,
pubmed-meshheading:15689381-Lysophospholipids,
pubmed-meshheading:15689381-Mesoderm,
pubmed-meshheading:15689381-Mice,
pubmed-meshheading:15689381-Mice, Transgenic,
pubmed-meshheading:15689381-Models, Biological,
pubmed-meshheading:15689381-Pancreas,
pubmed-meshheading:15689381-Phenotype,
pubmed-meshheading:15689381-RNA,
pubmed-meshheading:15689381-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15689381-Sepharose,
pubmed-meshheading:15689381-Sphingosine,
pubmed-meshheading:15689381-Time Factors
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| pubmed:year |
2005
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| pubmed:articleTitle |
Vascular function and sphingosine-1-phosphate regulate development of the dorsal pancreatic mesenchyme.
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| pubmed:affiliation |
Department of Medical Biochemistry, Box 440, Göteborg University, S-405 30 Göteborg, Sweden.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|