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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-2-2
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pubmed:abstractText |
Oligonucleotide microarrays provide a high-throughput method for exploring genomes. In addition to their utility for gene-expression analysis, oligonucleotide-expression arrays have also been used to perform genotyping on genomic DNA. Here, we show that in segregants from a cross between two unrelated strains of Saccharomyces cerevisiae, high-quality genotype data can also be obtained when mRNA is hybridized to an oligonucleotide-expression array. We were able to identify and genotype nearly 1000 polymorphisms at an error rate close to 3% in segregants and at an error rate of 7% in diploid strains, a performance comparable to methods using genomic DNA. In addition, we demonstrate how simultaneous genotyping and gene-expression profiling can reveal cis-regulatory variation by screening hundreds of genes for allele-specific expression. With this method, we discovered 70 ORFs with evidence for preferential expression of one allele in a diploid hybrid of two S. cerevisiae strains.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-11923494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12183620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12410233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12582260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12618383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12627232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12646905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12794640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-12902379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-15049300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-15306793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-15514893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15687292-9712584
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1088-9051
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
284-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15687292-Alleles,
pubmed-meshheading:15687292-DNA, Fungal,
pubmed-meshheading:15687292-Gene Expression Profiling,
pubmed-meshheading:15687292-Gene Expression Regulation, Fungal,
pubmed-meshheading:15687292-Genes, Fungal,
pubmed-meshheading:15687292-Genome, Fungal,
pubmed-meshheading:15687292-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15687292-Research Design,
pubmed-meshheading:15687292-Saccharomyces cerevisiae,
pubmed-meshheading:15687292-Species Specificity
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pubmed:year |
2005
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pubmed:articleTitle |
Simultaneous genotyping, gene-expression measurement, and detection of allele-specific expression with oligonucleotide arrays.
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pubmed:affiliation |
Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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