Linked Life Data

15687100

Source:http://linkedlifedata.com/resource/pubmed/id/15687100

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-5-10
pubmed:abstractText
Although starvation-induced biochemical and metabolic changes are perceived by the hypothalamus, the adrenal gland plays a key role in the integration of metabolic activity and energy balance, implicating feeding as a major synchronizer of rhythms in the hypothalamic-pituitary-adrenal (HPA) axis. Given that orexins are involved in regulating food intake and activating the HPA axis, we hypothesized that food deprivation, an acute challenge to the systems that regulate energy balance, should elicit changes in orexin receptor signaling at the hypothalamic and adrenal levels. Food deprivation induced orexin type 1 (OX1R) and 2 (OX2R) receptors at mRNA and protein levels in the hypothalamus, in addition to a fivefold increase in prepro-orexin mRNA. Cleaved peptides OR-A and OR-B are also elevated at the protein level. Interestingly, adrenal OX1R and OX2R levels were significantly reduced in food-deprived animals, whereas there was no expression of prepro-orexin in the adrenal gland in either state. Food deprivation exerted a differential effect on OXR-G protein coupling. In the hypothalamus of food deprived rats compared with controls, a significant increase in coupling of orexin receptors to Gq, Gs, and Go was demonstrated, whereas coupling to Gi was relatively less. However, in the adrenal cortex of the food-deprived animal, there was decreased coupling of orexin receptors to Gs, Go, and Gq and increased coupling to Gi. Subsequent second-messenger studies (cAMP/IP3) have supported these findings. Our data indicate that food deprivation has differential effects on orexin receptor expression and their signaling characteristics at the hypothalamic and adrenocortical levels. These findings suggest orexins as potential metabolic regulators within the HPA axis both centrally and peripherally.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0193-1849
pubmed:author
pubmed:issnType
Print
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E1089-100
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15687100-Adrenal Cortex, pubmed-meshheading:15687100-Animals, pubmed-meshheading:15687100-Blotting, Western, pubmed-meshheading:15687100-Cholera Toxin, pubmed-meshheading:15687100-Corticosterone, pubmed-meshheading:15687100-Food Deprivation, pubmed-meshheading:15687100-GTP-Binding Proteins, pubmed-meshheading:15687100-Gene Expression Regulation, pubmed-meshheading:15687100-Hypothalamo-Hypophyseal System, pubmed-meshheading:15687100-Hypothalamus, pubmed-meshheading:15687100-Male, pubmed-meshheading:15687100-Pertussis Toxin, pubmed-meshheading:15687100-Pituitary-Adrenal System, pubmed-meshheading:15687100-RNA, Messenger, pubmed-meshheading:15687100-Random Allocation, pubmed-meshheading:15687100-Rats, pubmed-meshheading:15687100-Rats, Wistar, pubmed-meshheading:15687100-Receptors, G-Protein-Coupled, pubmed-meshheading:15687100-Receptors, Neuropeptide, pubmed-meshheading:15687100-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15687100-Signal Transduction
pubmed:year
2005
pubmed:articleTitle
Food deprivation differentially modulates orexin receptor expression and signaling in rat hypothalamus and adrenal cortex.
pubmed:affiliation
Biomedical Research Institute, Univ. of Warwick, Gibbet Hill Rd., Coventry CV4 7AL, UK. e.k.karteris@warwick.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't