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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-2-1
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pubmed:abstractText |
The exact role of T-helper (Th) cells that precede the clinical manifestation of type 1 diabetes remains unclear. The aim of this investigation was to study the Th1- and Th2-like profile in children and adults with high risk of developing the disease. Peripheral blood mononuclear cells were collected from high-risk children and adults and from healthy individuals matched for age and gender. Using the sensitive enzyme-linked immunospot (ELISPOT) technique to divide Th1- from Th2-like lymphocytes, secretion of interferon-gamma (IFN-gamma) and interleukin-4 was analysed from lymphocytes spontaneously and after in vitro stimulation with different antigens, based on present paradigms regarding the pathogenesis of type 1 diabetes. Compared to the response observed in healthy individuals, we found that individuals with a high risk of developing type 1 diabetes, especially children, responded with less IFN-gamma secretion to the three autoantigens glutamic acid decarboxylase 65 (GAD65), insulin and tyrosinphosphatase (IA-2). Thus, a diminished Th1-like response by in vitro autoantigen stimulation was observed in especially children with a high risk of developing type 1 diabetes. Reduced Th1/Th2 response was related to signs of beta cell exhaustion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15683454-Adolescent,
pubmed-meshheading:15683454-Adult,
pubmed-meshheading:15683454-Autoantigens,
pubmed-meshheading:15683454-C-Reactive Protein,
pubmed-meshheading:15683454-Child,
pubmed-meshheading:15683454-Diabetes Mellitus, Type 1,
pubmed-meshheading:15683454-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15683454-Female,
pubmed-meshheading:15683454-Glutamate Decarboxylase,
pubmed-meshheading:15683454-Humans,
pubmed-meshheading:15683454-Insulin,
pubmed-meshheading:15683454-Interferon-gamma,
pubmed-meshheading:15683454-Interleukin-4,
pubmed-meshheading:15683454-Lymphocyte Activation,
pubmed-meshheading:15683454-Male,
pubmed-meshheading:15683454-Middle Aged,
pubmed-meshheading:15683454-Niacinamide,
pubmed-meshheading:15683454-Prediabetic State,
pubmed-meshheading:15683454-Proinsulin,
pubmed-meshheading:15683454-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:15683454-Protein Tyrosine Phosphatases,
pubmed-meshheading:15683454-Th1 Cells,
pubmed-meshheading:15683454-Th2 Cells
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pubmed:year |
2005
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pubmed:articleTitle |
Diminished Th1-like response to autoantigens in children with a high risk of developing type 1 diabetes.
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pubmed:affiliation |
Division of Paediatrics and Diabetes Research Centre, Department of Molecular & Clinical Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden. maria.faresjo@imk.liu.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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