Linked Life Data

15682289

Source:http://linkedlifedata.com/resource/pubmed/id/15682289

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-10-27
pubmed:abstractText
3-Hydroxypropionaldehyde (3-HPA) produced by Lactobacillus reuteri is a broad-spectrum antimicrobial substance of glycerol conversion. The aim of the present work was to optimize 3-HPA production by Lb. reuteri ATCC 53608 using a two-step process. The first step was the production of Lb. reuteri cells in optimal conditions. Cells were then harvested by centrifugation and suspended in glycerol solution, which the resting cells bioconverted to 3-HPA. The effect of biomass concentration, temperature, glycerol concentration, anaerobic/micro-aerophilic conditions, and incubation time was studied for high 3-HPA production. 3-HPA accumulation was limited by the death of cells in contact with high concentrations of 3-HPA. However, a very high 3-HPA concentration of 235+/-3 mM was obtained after 45 min of incubation at 30 degrees C in 400 mM glycerol for an initial free-cell concentration of 1.6+/-0.3x10(10) viable cells/ml. A high viability was maintained at low temperatures in the range 5-15 degrees C, but with a slightly lower yield of 3-HPA at 5 degrees C compared with higher temperatures, up to 37 degrees C. Successive 1-h incubations of Lb. reuteri cells in 200 mM glycerol at 15 degrees C to tentatively reuse the cells resulted in decreasing 3-HPA concentrations at the end of each cycle, with two successful production cycles yielding high 3-HPA concentrations of 147+/-1 mM and 128+/-2 mM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-74
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Production of 3-hydroxypropionaldehyde using a two-step process with Lactobacillus reuteri.
pubmed:affiliation
Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology, ETH Zentrum, Schmelzbergstrasse 9, Zurich, 8092, Switzerland.
pubmed:publicationType
Journal Article