Linked Life Data

15681056

Source:http://linkedlifedata.com/resource/pubmed/id/15681056

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-1-31
pubmed:abstractText
A new simple and efficient method for isolation of intact WSSV viral particles from infected crayfish tissues with high yield was developed. Abundant viral particles could be obtained with only a few steps of conventional differential centrifugations, while no density gradient centrifugation or ultracentrifugation was required. The concentrated virus preparations were further studied by transmission electron microscopy and polyacrylamide gel electrophoresis. Using negative-staining TEM, we found that purified viral particles were coated with integral envelope. At least 23 major structural proteins from purified WSSV virions could be observed by SDS-PAGE. By this method, about 10(12) viral particles could be recovered from 10 g of infected crayfish tissues. Moreover, purified virus does not lose its biological activity. Using purified virus, the minimal amount of WSSV that could initiate a successful virus proliferation in crayfish was determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
A simple and efficient method for purification of intact white spot syndrome virus (WSSV) viral particles.
pubmed:affiliation
Key Laboratory of Marine Biogenetic Resources (SOA and FJ), Third Institute of Oceanography, SOA. 178 Daxue Rd., Xiamen 361005, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't