Linked Life Data

15680490

Source:http://linkedlifedata.com/resource/pubmed/id/15680490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2005-1-31
pubmed:abstractText
Excitation of sensory nerves and activation of myocardial protein kinase C (PKC) epsilon contribute to the transduction of remote preconditioning (RPC) to the heart. Since calcitonin gene related peptide (CGRP) is an important mediator of sensory neurons we tried to delineate whether CGRP a) protects the heart from ischemic injury, b) is involved in cardioprotection after RPC, and c) leads to an activation of myocardial PKCepsilon. RPC was achieved by brief mesenteric artery occlusion followed by reperfusion. Myocardial infarct size (IS) was measured by TTC staining after temporary coronary artery occlusion (CAO) in rats. CGRP plasma levels were determined by radioimmunoassay and PKCepsilon was measured by quantitative immunoblotting. CGRP infusion reduced infarct size by 57%, an action that was abolished after co-treatment with the PKC inhibitor chelerythrine. RPC significantly increased CGRP plasma levels, reduced infarct size, and activated myocardial PKCepsilon. Infarct size reduction was abolished and PKCepsilon activation was significantly attenuated by CGRP(8-37), a specific CGRP receptor antagonist. Ganglion blockade with hexamethonium did not influence CGRP release by RPC but abolished CGRP mediated myocardial PKCepsilon activation. In conclusion, CGRP protects the heart from ischemic injury and is involved in RPC, presumably by activating myocardial PKCepsilon.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0167-0115
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-24
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15680490-Animals, pubmed-meshheading:15680490-Blood Pressure, pubmed-meshheading:15680490-Calcitonin Gene-Related Peptide, pubmed-meshheading:15680490-Cardiotonic Agents, pubmed-meshheading:15680490-Dose-Response Relationship, Drug, pubmed-meshheading:15680490-Enzyme Activation, pubmed-meshheading:15680490-Heart, pubmed-meshheading:15680490-Ischemic Preconditioning, Myocardial, pubmed-meshheading:15680490-Male, pubmed-meshheading:15680490-Myocardial Infarction, pubmed-meshheading:15680490-Myocardial Ischemia, pubmed-meshheading:15680490-Neurons, Afferent, pubmed-meshheading:15680490-Protein Kinase C, pubmed-meshheading:15680490-Protein Kinase C-epsilon, pubmed-meshheading:15680490-Random Allocation, pubmed-meshheading:15680490-Rats, pubmed-meshheading:15680490-Rats, Wistar
pubmed:year
2005
pubmed:articleTitle
Calcitonin gene related peptide mediates cardioprotection by remote preconditioning.
pubmed:affiliation
Institute of experimental and clinical Pharmacology and Toxicology, Medical University of Schleswig Holstein, Campus Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany. wolfrum@medinf-mu.luebeck.de
pubmed:publicationType
Journal Article