Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-5-26
pubmed:abstractText
Addition of high concentrations of compatible co-solutes such as sugars, sugar alcohols and polyols has recently been shown to lead to marked increases in the thermal stability of oxygen-evolution in chloroplasts (Williams et al. (1992) Biochim. Biophys. Acta 1099, 137-144). In this paper, a similar stabilisation is demonstrated for oxygen-evolving PS II core preparations. The presence of such co-solutes appears, however, to have no ability to stabilise PS II reaction-centre preparations against heat-induced changes in their absorption spectrum. Nor do they protect electron transport from artificial electron donors in PS II core preparations lacking the extrinsic 33 kDa polypeptide of the oxygen-evolution system. Measurements performed on core preparations retaining the 33 kDa polypeptide but lacking the 17 kDa and 23 kDa polypeptides indicate that the co-solutes protect PS-II-mediated electron transport by stabilising the binding of the 33 kDa polypeptide to the core complexes. These findings are discussed in terms of an extension of the general principles underlying the Hofmeister effect observed for soluble proteins to the stabilisation of photosynthetic membrane preparations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
1100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
92-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Stabilisation of PS-II-mediated electron transport in oxygen-evolving PS II core preparations by the addition of compatible co-solutes.
pubmed:affiliation
Biomolecular Sciences Division, King's College London, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't