Linked Life Data

1567856

Source:http://linkedlifedata.com/resource/pubmed/id/1567856

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1992-5-28
pubmed:databankReference
pubmed:abstractText
The ME1a1 and ME1a2 elements are cis-acting DNA sequences that exist at positions -46 and -85, respectively, within the P2 promoter of the c-myc gene. These elements are required for optimal transcription initiation from P2. The proteins that bind to these elements are identical or very similar. Here we have isolated a cDNA clone that encodes the carboxy-terminal 494 amino acids of the human ME1a1/ME1a2 factor. This factor is referred to as "ZF87" (zinc-finger protein, 87 kilodaltons). ZF87 specifically binds the ME1a1 element with higher affinity than the ME1a2 element. Western blot analysis indicates that the full-length protein has a relative molecular mass of approximately 87,000 daltons, and Northern blot analysis shows that it is encoded by a 5-kb transcript. ZF87 contains six zinc-finger domains, of the Cys2-His2 type, at the carboxy terminus of the protein. The protein also contains extended tracts of polyalanine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
31
pubmed:geneSymbol
c-myc
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4102-10
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Isolation of a novel cDNA encoding a zinc-finger protein that binds to two sites within the c-myc promoter.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't