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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2005-3-28
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pubmed:abstractText |
Heat shock (HS) activates mitogen-activated protein (MAP) kinases. Although prior exposure to nonlethal HS makes cells refractory to the lethal effect of a subsequent HS, it is unclear whether this also occurs in MAP kinase activation. This study was undertaken to evaluate the effect of a heat pretreatment on MAP kinase activation by a subsequent HS and to elucidate its possible mechanism. Preheating did not make BEAS-2B cells refractory to extracellular signal-regulated protein kinase (ERK) and c-Jun N-terminal kinase (JNK) activation by a second HS but accelerated their inactivation after HS. The rapid inactivation of ERK and JNK was dependent on de novo protein synthesis and associated with the up-regulation of heat shock protein 70 (HSP70). Moreover, the inhibition of phosphatase activity reversed this rapid inactivation. MAP kinase phosphatase-1 (MKP-1) expression was increased by HS, and the presence of its phosphorylated form (p-MKP-1) correlated with the observed rapid ERK and JNK inactivation. Blocking induction of p-MKP-1 with antisense MKP-1 oligonucleotides suppressed the rapid inactivation of ERK and JNK in preheated cells. HSP70 overexpression caused the early phosphorylation of MKP-1. Moreover, MKP-1 phosphorylation and the rapid inactivation of ERK were inhibited by blocking HSP70 induction in preheated cells. In addition, MKP-1 was insolubilized by HS, and HSP70 associated physically with MKP-1, suggesting that a chaperone effect of HSP70 might have caused the early phosphorylation of MKP-1. These results indicate that preheating accelerated MAP kinase inactivation after a second HS and that this is related to a HSP70-mediated increase in p-MKP-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DUSP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Dual Specificity Phosphatase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Dusp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/HSP70 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13179-86
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15677475-Adenoviridae,
pubmed-meshheading:15677475-Animals,
pubmed-meshheading:15677475-Blotting, Western,
pubmed-meshheading:15677475-Bronchi,
pubmed-meshheading:15677475-Cell Cycle Proteins,
pubmed-meshheading:15677475-Cells, Cultured,
pubmed-meshheading:15677475-Dual Specificity Phosphatase 1,
pubmed-meshheading:15677475-Enzyme Inhibitors,
pubmed-meshheading:15677475-Epithelial Cells,
pubmed-meshheading:15677475-HSP70 Heat-Shock Proteins,
pubmed-meshheading:15677475-Hot Temperature,
pubmed-meshheading:15677475-Humans,
pubmed-meshheading:15677475-Immediate-Early Proteins,
pubmed-meshheading:15677475-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:15677475-MAP Kinase Kinase 4,
pubmed-meshheading:15677475-MAP Kinase Signaling System,
pubmed-meshheading:15677475-Mice,
pubmed-meshheading:15677475-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:15677475-NIH 3T3 Cells,
pubmed-meshheading:15677475-Oligonucleotides,
pubmed-meshheading:15677475-Oligonucleotides, Antisense,
pubmed-meshheading:15677475-Phosphoprotein Phosphatases,
pubmed-meshheading:15677475-Phosphorylation,
pubmed-meshheading:15677475-Protein Binding,
pubmed-meshheading:15677475-Protein Phosphatase 1,
pubmed-meshheading:15677475-Protein Tyrosine Phosphatases,
pubmed-meshheading:15677475-Time Factors,
pubmed-meshheading:15677475-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
Preheating accelerates mitogen-activated protein (MAP) kinase inactivation post-heat shock via a heat shock protein 70-mediated increase in phosphorylated MAP kinase phosphatase-1.
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pubmed:affiliation |
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Korea.
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pubmed:publicationType |
Journal Article
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