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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-5-20
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pubmed:abstractText |
Using our enzyme immunoassay system developed for recombinant hNGF, we examined the synthesis and secretion of human NGF (hNGF) by human fibroblast (WS-1) cells. The amount of the factor secreted by WS-1 cells increased linearly and a significant amount of NGF was detected in the conditioned medium of WS-1 cultures. WS-1 NGF showed properties identical to those of recombinant human NGF in immunoreactivity and molecular weight. An increase in cell density or the withdrawal of serum from the culture medium caused a drastic decrease in the rate of NGF secretion. These results suggest that WS-1 cells are able to synthesize and secrete hNGF in culture and that the synthesis/secretion is regulated in a growth phase-dependent manner.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
373-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1567443-Cell Division,
pubmed-meshheading:1567443-Cell Line,
pubmed-meshheading:1567443-Chromatography, Gel,
pubmed-meshheading:1567443-Fibroblasts,
pubmed-meshheading:1567443-Humans,
pubmed-meshheading:1567443-Immunoenzyme Techniques,
pubmed-meshheading:1567443-Kinetics,
pubmed-meshheading:1567443-Molecular Weight,
pubmed-meshheading:1567443-Nerve Growth Factors,
pubmed-meshheading:1567443-Recombinant Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
Human fibroblast cells synthesize and secrete nerve growth factor in culture.
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pubmed:affiliation |
Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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