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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-5-20
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pubmed:abstractText |
L-Asparaginase from Escherichia coli, an anti-tumor enzyme, was chemically modified with two types of maleic anhydride copolymers with a comb-shaped form, the one composed of polyoxyethylene allyl methyl diether with the molecular weight of 13,000 (activated PM13) and the other of polyoxyethylene 2-methyl-2-propenyl methyl diether with 100,000 (activated PM100). The modified asparaginases (PM13- and PM100-asparaginases) exhibited the complete loss of immunoreactivity towards anti-asparaginase serum. The enzymic activity of PM100-asparaginase without immunoreactivity was well retained by 85% of non-modified one, while that of PM13-asparaginase was retained 46%. These results were discussed in relation to the chemical structure of modifying reagents including chain shaped-polyethylene glycol derivatives.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Asparaginase,
http://linkedlifedata.com/resource/pubmed/chemical/Maleic Anhydrides,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Trinitrobenzenesulfonic Acid
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
144-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1567419-Amino Acids,
pubmed-meshheading:1567419-Asparaginase,
pubmed-meshheading:1567419-Escherichia coli,
pubmed-meshheading:1567419-Kinetics,
pubmed-meshheading:1567419-Maleic Anhydrides,
pubmed-meshheading:1567419-Molecular Conformation,
pubmed-meshheading:1567419-Polyethylene Glycols,
pubmed-meshheading:1567419-Spectrophotometry, Ultraviolet,
pubmed-meshheading:1567419-Trinitrobenzenesulfonic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Chemical modification of L-asparaginase with a comb-shaped copolymer of polyethylene glycol derivative and maleic anhydride.
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pubmed:affiliation |
Department of Materials Science and Technology, Toin University of Yokohama, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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