Linked Life Data

15672380

Source:http://linkedlifedata.com/resource/pubmed/id/15672380

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-2-23
pubmed:abstractText
Potentially damaging reactive oxygen species (ROS) are involved in a number of pathways ranging from signal transduction to apoptosis. Cells have adapted this alteration in redox status into a complex regulatory mechanism. ROS are specifically able to induce the expression of a multitude of genes. We constructed and characterized "oxidative stress probes" consisting of promoter fusions of several ROS-induced genes and the green fluorescent protein (GFP) reporter gene. Specifically, the sodA, fumC, zwf, acnA, acrAB, and soxS genes from the SoxRS regulon and the katG and ahpC genes from OxyR regulon, which respond to the superoxide anion and hydrogen peroxide, were studied. Our results revealed not only different levels of background transcription, but different induction levels both in terms of timing and strength. These systematic studies were performed under a uniform parallel platform and have provided insight into the complicated gene regulation of the oxidative stress regulons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3592
pubmed:author
pubmed:copyrightInfo
2005 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
574-87
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Quantitative and kinetic study of oxidative stress regulons using green fluorescent protein.
pubmed:affiliation
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't