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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-5-15
|
| pubmed:abstractText |
Sepharose CL-6B column chromatography of crude extracts from the slices of regenerating rat livers after partial hepatectomy and sham-operated controls labeled with [35S]sulfuric acid revealed an enhancement of [35S]sulfate incorporation into proteoglycan fractions during regeneration. The 35S-labeled proteoglycans contained heparan sulfate (more than 80% of the total) and chondroitin/dermatan sulfate. The 35S-incorporation into both glycosaminoglycans increased to maxima 3-5 days after partial hepatectomy and decreased thereafter toward the respective control levels. When [35S]sulfuric acid was replaced by [3H]glucosamine, similar results were obtained. These results suggest that the maximal stimulation of proteoglycan synthesis in regenerating rat liver follows the maximal mitosis of hepatic cells 1-2 days after partial hepatectomy. The 35S-labeled proteoglycans from regenerating liver 3 days after partial hepatectomy and control were analyzed further. They were similar in chromatographic behavior on a gel filtration or an anion-exchange column and in glycosaminoglycan composition. Their glycosaminoglycans were indistinguishable in electrophoretic mobility. However, these proteoglycans were slightly but significantly different in their affinity to octyl-Sepharose and in the molecular-weight distribution of their glycosaminoglycans.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glucosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosaminoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfates,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0003-9861
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
294
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
544-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1567211-Animals,
pubmed-meshheading:1567211-Chromatography, High Pressure Liquid,
pubmed-meshheading:1567211-Chromatography, Ion Exchange,
pubmed-meshheading:1567211-Glucosamine,
pubmed-meshheading:1567211-Glycoproteins,
pubmed-meshheading:1567211-Glycosaminoglycans,
pubmed-meshheading:1567211-Kinetics,
pubmed-meshheading:1567211-Liver,
pubmed-meshheading:1567211-Liver Regeneration,
pubmed-meshheading:1567211-Male,
pubmed-meshheading:1567211-Oligosaccharides,
pubmed-meshheading:1567211-Proteoglycans,
pubmed-meshheading:1567211-Rats,
pubmed-meshheading:1567211-Rats, Inbred Strains,
pubmed-meshheading:1567211-Reference Values,
pubmed-meshheading:1567211-Sulfates,
pubmed-meshheading:1567211-Sulfur Radioisotopes,
pubmed-meshheading:1567211-Time Factors,
pubmed-meshheading:1567211-Tritium
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Alteration of rat liver proteoglycans during regeneration.
|
| pubmed:affiliation |
Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|