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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-1-26
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pubmed:abstractText |
Cigarette-induced endothelial dysfunction could be an early mediator of atherosclerosis. In this study, we explored the mechanisms of cigarette smoke extract (CSE)-induced human aortic endothelial cells (HAEC) apoptosis. We found that 10-65% of HAECs underwent apoptotic changes when HAECs were exposed to 0.001-0.02 cigarette equivalent unit of CSE for 4 h. CSE activated the caspases-3 and 8, the p38 MAP kinase and stress activated protein kinase/c-Jun N-terminal protein kinase (SAPK/JNK). Specific inhibitors of p38 MAP or SAPK/JNK reduced CSE-induced caspase activation. We further showed that eNOS pre-activation by L-arginine reduced endothelial apoptosis from 65% to 5%; and eNOS inhibition by N-omega-nitro-L-arginine methyl ester accentuated CSE-induced endothelial apoptosis. We suggest that appropriate endogenous NO production may be an important protective mechanism against smoking-induced endothelial damage.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-10232046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-10653649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-10753895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-10760510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-11134047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-11161833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-11162777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-11305508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-11524431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-12244325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-12547733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-12801522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-14627517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-15105295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-1654842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-3010861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-6134041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7517402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7526779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7535247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7540548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7622868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7706495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7890697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-7929318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-8026008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-8044585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-8137421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-8643472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-8944624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9171946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9186585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9242400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9388267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9388494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9441900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9466966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9647775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9738946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9755110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9780184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15670837-9806747
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NOS3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type III
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
579
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
733-40
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15670837-Humans,
pubmed-meshheading:15670837-Smoking,
pubmed-meshheading:15670837-Tobacco,
pubmed-meshheading:15670837-Nitric Oxide,
pubmed-meshheading:15670837-Base Sequence,
pubmed-meshheading:15670837-Cells, Cultured,
pubmed-meshheading:15670837-Endothelium, Vascular,
pubmed-meshheading:15670837-Apoptosis,
pubmed-meshheading:15670837-DNA Primers,
pubmed-meshheading:15670837-Blotting, Western,
pubmed-meshheading:15670837-Nitric Oxide Synthase,
pubmed-meshheading:15670837-Mitogen-Activated Protein Kinases
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