Source:http://linkedlifedata.com/resource/pubmed/id/15668976
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2005-1-31
|
| pubmed:abstractText |
The causative pathomechanism of sporadic amyotrophic lateral sclerosis (ALS) is not clearly understood. Using microarray technology combined with laser-captured microdissection, gene expression profiles of degenerating spinal motor neurons isolated from autopsied patients with sporadic ALS were examined. Gene expression was quantitatively assessed by real-time reverse transcription polymerase chain reaction and in situ hybridization. Spinal motor neurons showed a distinct gene expression profile from the whole spinal ventral horn. Three percent of genes examined were downregulated, and 1% were upregulated in motor neurons. Downregulated genes included those associated with cytoskeleton/axonal transport, transcription, and cell surface antigens/receptors, such as dynactin, microtubule-associated proteins, and early growth response 3 (EGR3). In contrast, cell death-associated genes were mostly upregulated. Promoters for cell death pathway, death receptor 5, cyclins A1 and C, and caspases-1, -3, and -9, were upregulated, whereas cell death inhibitors, acetyl-CoA transporter, and NF-kappaB were also upregulated. Moreover, neuroprotective neurotrophic factors such as ciliary neurotrophic factor (CNTF), Hepatocyte growth factor (HGF), and glial cell line-derived neurotrophic factor were upregulated. Inflammation-related genes, such as those belonging to the cytokine family, were not, however, significantly upregulated in either motor neurons or ventral horns. The motor neuron-specific gene expression profile in sporadic ALS can provide direct information on the genes leading to neurodegeneration and neuronal death and are helpful for developing new therapeutic strategies.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0364-5134
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| pubmed:author |
pubmed-author:AdachiHiroakiH,
pubmed-author:DoyuManabuM,
pubmed-author:HashizumeYoshioY,
pubmed-author:IshigakiShinsukeS,
pubmed-author:JiangYue-MeiYM,
pubmed-author:KatsunoMasahisaM,
pubmed-author:KobayashiYasushiY,
pubmed-author:LiangYidengY,
pubmed-author:NiwaJun-ichiJ,
pubmed-author:SobueGenG,
pubmed-author:TakeuchiHideyukiH,
pubmed-author:TanakaFumiakiF,
pubmed-author:TeraoShinichiS,
pubmed-author:YamamotoMasahikoM,
pubmed-author:YoshidaMariM,
pubmed-author:YoshiharaTsuyoshiT
|
| pubmed:issnType |
Print
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
236-51
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15668976-Adult,
pubmed-meshheading:15668976-Aged,
pubmed-meshheading:15668976-Aged, 80 and over,
pubmed-meshheading:15668976-Amyotrophic Lateral Sclerosis,
pubmed-meshheading:15668976-Anterior Horn Cells,
pubmed-meshheading:15668976-DNA Primers,
pubmed-meshheading:15668976-Female,
pubmed-meshheading:15668976-Gene Expression Profiling,
pubmed-meshheading:15668976-Humans,
pubmed-meshheading:15668976-Immunohistochemistry,
pubmed-meshheading:15668976-In Situ Hybridization,
pubmed-meshheading:15668976-Lasers,
pubmed-meshheading:15668976-Male,
pubmed-meshheading:15668976-Microdissection,
pubmed-meshheading:15668976-Middle Aged,
pubmed-meshheading:15668976-Motor Neurons,
pubmed-meshheading:15668976-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15668976-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15668976-Spinal Cord
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Gene expression profile of spinal motor neurons in sporadic amyotrophic lateral sclerosis.
|
| pubmed:affiliation |
Department of Neurology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|