Linked Life Data

15668173

Source:http://linkedlifedata.com/resource/pubmed/id/15668173

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-1-25
pubmed:abstractText
Accurate chromosome segregation depends on proper assembly and function of the kinetochore and the mitotic spindle. In the budding yeast, Saccharomyces cerevisiae, the highly conserved protein kinase Mps1 has well-characterized roles in spindle pole body (SPB, yeast centrosome equivalent) duplication and the mitotic checkpoint. However, an additional role for Mps1 is suggested by phenotypes of MPS1 mutations that include genetic interactions with kinetochore mutations and meiotic chromosome segregation defects and also by the localization of Mps1 at the kinetochore, the latter being independent of checkpoint activation. We have developed a new MPS1 allele, mps1-as1, that renders the kinase specifically sensitive to a cell-permeable ATP analog inhibitor, allowing us to perform high-resolution execution point experiments that identify a novel role for Mps1 subsequent to SPB duplication. We demonstrate, by using both fixed- and live-cell fluoresence techniques, that cells lacking Mps1 function show severe defects in mitotic spindle formation, sister kinetochore positioning at metaphase, and chromosome segregation during anaphase. Taken together, our experiments are consistent with an important role for Mps1 at the kinetochore in mitotic spindle assembly and function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0960-9822
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
160-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15668173-Amino Acid Sequence, pubmed-meshheading:15668173-Blotting, Western, pubmed-meshheading:15668173-CDC28 Protein Kinase, S cerevisiae, pubmed-meshheading:15668173-Chromosome Segregation, pubmed-meshheading:15668173-Flow Cytometry, pubmed-meshheading:15668173-Fluorescent Antibody Technique, pubmed-meshheading:15668173-Kinetochores, pubmed-meshheading:15668173-Microscopy, Electron, pubmed-meshheading:15668173-Mitosis, pubmed-meshheading:15668173-Mitotic Spindle Apparatus, pubmed-meshheading:15668173-Molecular Sequence Data, pubmed-meshheading:15668173-Mutation, pubmed-meshheading:15668173-Protein-Serine-Threonine Kinases, pubmed-meshheading:15668173-Protein-Tyrosine Kinases, pubmed-meshheading:15668173-Saccharomyces cerevisiae, pubmed-meshheading:15668173-Saccharomyces cerevisiae Proteins, pubmed-meshheading:15668173-Sequence Alignment
pubmed:year
2005
pubmed:articleTitle
Chemical genetics reveals a role for Mps1 kinase in kinetochore attachment during mitosis.
pubmed:affiliation
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309-0347, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.