Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2005-5-19
pubmed:abstractText
The lack of comparable metabolic state assays severely limits understanding the metabolic changes caused by genetic or environmental perturbations. The present study reports the application of a novel derivatization method for metabolome analysis of yeast, coupled to data-mining software that achieve comparable throughput, effort and cost compared with DNA arrays. Our sample workup method enables simultaneous metabolite measurements throughout central carbon metabolism and amino acid biosynthesis, using a standard GC-MS platform that was optimized for this purpose. As an implementation proof-of-concept, we assayed metabolite levels in two yeast strains and two different environmental conditions in the context of metabolic pathway reconstruction. We demonstrate that these differential metabolite level data distinguish among sample types, such as typical metabolic fingerprinting or footprinting. More importantly, we demonstrate that this differential metabolite level data provides insight into specific metabolic pathways and lays the groundwork for integrated transcription-metabolism studies of yeasts.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-10022859, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-10191379, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-10929108, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-11382363, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-11418108, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12000970, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12064328, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12176828, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12415116, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12566402, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12590111, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12590120, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12740584, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-12788545, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14554197, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14555958, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14578455, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14597658, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14705791, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14730673, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14748081, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-14985762, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-15136733, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-1514678, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-1523884, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-2202777, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-3106975, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-3517877, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-8224185, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-8944223, http://linkedlifedata.com/resource/pubmed/commentcorrection/15667247-9744112
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1470-8728
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
388
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
669-77
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
High-throughput metabolic state analysis: the missing link in integrated functional genomics of yeasts.
pubmed:affiliation
Centre for Microbial Biotechnology, Technical University of Denmark, BioCentrum-DTU, Building 223, DK-2800 Kongens Lyngby, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't