Source:http://linkedlifedata.com/resource/pubmed/id/15664636
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
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pubmed:dateCreated |
2005-1-24
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pubmed:abstractText |
Recent evidence suggests that following visible-light (VL) irradiation, CQ and the CQ-related photosensitizers benzil (BZ), benzophenone (BP), and 9-fluorenone (9-F) generate initiating radicals that may indiscriminately react with molecular oxygen forming reactive oxygen species (ROS). The purpose of this investigation was to determine whether VL-irradiated CQ, BZ, BP, and 9-F cause DNA damage due to the generation of ROS in vitro. ROS formation by CQ and CQ-related photosensitizers+/-dimethyl-p-toluidine (DMT) was investigated in a cell-free system with VL irradiation. DNA damage was determined using PhiX-174 RF I supercoiled double-stranded plasmid DNA and ROS quantified with 4-((9-acridinecarbonyl)amino)-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO-9-AC), a fluorogenic ROS-sensitive probe. VL-irradiated CQ, BZ, BP, and 9-F (+/-DMT) produced significant DNA damage at 0.1, 0.5, and 1.0 mM and in a concentration-dependent manner (p<0.05). TEMPO-9-AC revealed that all investigated VL-irradiated photosensitizers produced significant amounts of ROS with BZ in the presence of DMT generating the most ROS after 30, 60, and 90 min. VL-irradiated CQ, BZ, BP, and 9-F +/-DMT continued to generate significant amounts of ROS 90 min after VL irradiation. As a result, future investigations should evaluate the effect of VL-irradiated photosensitizers in cells and possible protective effects provided by antioxidants.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/9-fluorenone,
http://linkedlifedata.com/resource/pubmed/chemical/Benzophenones,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorenes,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylglyoxal,
http://linkedlifedata.com/resource/pubmed/chemical/Photosensitizing Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Terpenes,
http://linkedlifedata.com/resource/pubmed/chemical/benzil,
http://linkedlifedata.com/resource/pubmed/chemical/benzophenone,
http://linkedlifedata.com/resource/pubmed/chemical/camphoroquinone
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0142-9612
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4091-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15664636-Benzophenones,
pubmed-meshheading:15664636-Biocompatible Materials,
pubmed-meshheading:15664636-DNA,
pubmed-meshheading:15664636-DNA Damage,
pubmed-meshheading:15664636-Fluorenes,
pubmed-meshheading:15664636-Light,
pubmed-meshheading:15664636-Materials Testing,
pubmed-meshheading:15664636-Oxidation-Reduction,
pubmed-meshheading:15664636-Phenylglyoxal,
pubmed-meshheading:15664636-Photosensitizing Agents,
pubmed-meshheading:15664636-Reactive Oxygen Species,
pubmed-meshheading:15664636-Terpenes
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pubmed:year |
2005
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pubmed:articleTitle |
The effect of camphorquinone (CQ) and CQ-related photosensitizers on the generation of reactive oxygen species and the production of oxidative DNA damage.
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pubmed:affiliation |
Department of Restorative Dentistry, University of Washington, D-770, Health Science Building, Box 357456, Seattle, WA 98195-7456, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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