Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-1-24
pubmed:abstractText
RP-1, a herbal preparation of Podophyllum hexandrum has already been reported to provide protection against whole body lethal gamma irradiation (10 Gy). It has also been reported to render radioprotection to germ cells during spermatogenesis. Present study was undertaken to unravel the cellular and molecular mechanism of action of RP-1 on testicular system in strain 'A' mice. Various antioxidant parameters such as thiol content, glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST) enzyme activity, lipid peroxidation (LPO) and total protein levels were investigated. Thiol content was seen to increase significantly (p < 0.05) in both RP-1 alone and RP-1 pretreated irradiated groups over the irradiated groups at 8, 16 and 24 h. Irradiation (10 Gy) significantly decreased GPx, GST and GR activity in comparison to untreated control but RP-1 treatment before irradiation significantly (p < 0.05) countered radiation-induced decrease in the activity of these enzymes. Radiation-induced LPO was also found to be reduced at all time intervals by RP-1 treatment before irradiation. As compared to irradiated group the protein content in testicular tissue was increased in RP-1 pretreated irradiated group at 4 and 16 h significantly (p < 0.05). Comets revealed by single-cell gel electrophoresis were significantly longer (p < 0.001) in irradiated mice than in unirradiated control. RP-1 treatment before irradiation, however, rendered significant increase (p < 0.05) in comet length over the corresponding control and irradiated group initially at 4 h but at later time points, this was reduced significantly (p < 0.01) as compared to the irradiated group. RP-1 treatment alone rendered shorter comets at 8, 16 and 24 h than irradiated groups (p < 0.001). This study implies that RP-1 offers radioprotection at biochemical and cytogenetic level by protecting antioxidant enzymes, reducing LPO and increasing thiol content.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15663198-Animals, pubmed-meshheading:15663198-Comet Assay, pubmed-meshheading:15663198-Gamma Rays, pubmed-meshheading:15663198-Glutathione Peroxidase, pubmed-meshheading:15663198-Glutathione Reductase, pubmed-meshheading:15663198-Glutathione Transferase, pubmed-meshheading:15663198-Lipid Peroxidation, pubmed-meshheading:15663198-Male, pubmed-meshheading:15663198-Mice, pubmed-meshheading:15663198-Mice, Inbred A, pubmed-meshheading:15663198-Phytotherapy, pubmed-meshheading:15663198-Plant Extracts, pubmed-meshheading:15663198-Plants, Medicinal, pubmed-meshheading:15663198-Podophyllum, pubmed-meshheading:15663198-Proteins, pubmed-meshheading:15663198-Radiation-Protective Agents, pubmed-meshheading:15663198-Spermatogenesis, pubmed-meshheading:15663198-Sulfhydryl Compounds, pubmed-meshheading:15663198-Time Factors, pubmed-meshheading:15663198-Whole-Body Irradiation
pubmed:year
2004
pubmed:articleTitle
Radioprotective mechanism of Podophyllum hexandrum during spermatogenesis.
pubmed:affiliation
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig S. K. Mazumdar Marg, Delhi, India.
pubmed:publicationType
Journal Article, Comparative Study