Linked Life Data

15659570

Source:http://linkedlifedata.com/resource/pubmed/id/15659570

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-3-23
pubmed:abstractText
Due to its widespread use and production, di-n-butyl phthalate (DBP) has become an environmental contaminant. It has been detected in a variety of environmental strata worldwide, including air, water, and soil. Also, monobutyl phthalate, the major metabolite of DBP, has been detected in a variety of human matrices. As a proven endocrine disruptive compound, DBP may contribute to global amphibian declines at much lower concentrations than tested thus far. We evaluated the effects of low concentrations of DBP on spermatogenesis in Xenopus laevis, the African clawed frog. Xenopus tadpoles were exposed to 0, 0.1, 0.5, 1.0, 5.0, or 10.0 ppm DBP, beginning at sexual differentiation (Nieuwkoop and Faber stage 52; 3 weeks of age) and continuing until 100% of controls metamorphosed (stage 66; 8 weeks of age). Upon necropsy at 33 weeks, 4-6% of DBP-treated frogs had only one testis, and 2-4% had retained oviducts. In all DBP treatment groups, seminiferous tubule diameter and the average number of germ cell nests per tubule were lower, and the number of tubules with no germ cells was significantly higher (p < 0.05). The percent of secondary spermatogonial cell nests significantly decreased (p < 0.05) in 1.0, 5.0, and 10.0 ppm groups. Several lesions occurred in DBP-exposed testes including denudation of germ cells, vacuolization of Sertoli cell cytoplasm, thickening of lamina propria of seminiferous tubules, and focal lymphocytic infiltration. Entire sections of testes containing almost exclusively mature spermatozoa were found in 1.0, 5.0, and 10.0 ppm DBP-exposed testes, indicating impairment of spermiation. Testicular hypoplasia and seminiferous tubular dysgenesis were also evident in DBP-treated frogs. Thus, subchronic exposure to low concentrations of DBP impairs spermatogenesis in Xenopus laevis frogs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1096-6080
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
394-407
pubmed:dateRevised
2010-9-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Subchronic exposure to low concentrations of di-n-butyl phthalate disrupts spermatogenesis in Xenopus laevis frogs.
pubmed:affiliation
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado 80523, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.