Linked Life Data

15659234

Source:http://linkedlifedata.com/resource/pubmed/id/15659234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-1-20
pubmed:databankReference
pubmed:abstractText
We cloned a rat BAALC 1-6-8 isoform cDNA (GenBank Accession No. AB073318) that encoded a 22-kDa protein, and identified endogenous BAALC 1-6-8 protein in the brain. The gene was expressed widely in the frontal part of the brain, and the protein was localized to the synaptic sites and was increased in parallel with synaptogenesis. The protein interacted with the alpha, but not beta, subunit of Ca(2+)/calmodulin-dependent protein kinase II (CaMKIIalpha). The interaction occurred between the N-terminal 35-amino-acid region of BAALC 1-6-8 protein and the C-terminal end of the regulatory domain of CaMKIIalpha, which contains alpha isoform-specific sequence. Thus, the interaction may be CaMKIIalpha-specific. We also found that BAALC 1-6-8 protein, as well as CaMKIIalpha, was localized to lipid rafts and that both myristoylation and palmitoylation of BAALC 1-6-8 N-terminal portion were required for targeting of the protein into lipid rafts. These findings suggest that BAALC 1-6-8 protein play a synaptic role at the postsynaptic lipid raft possibly through interaction with CaMKIIalpha.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
647-59
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15659234-Alternative Splicing, pubmed-meshheading:15659234-Amino Acid Sequence, pubmed-meshheading:15659234-Animals, pubmed-meshheading:15659234-Base Sequence, pubmed-meshheading:15659234-Blotting, Western, pubmed-meshheading:15659234-Brain, pubmed-meshheading:15659234-COS Cells, pubmed-meshheading:15659234-Calcium-Calmodulin-Dependent Protein Kinase Type 2, pubmed-meshheading:15659234-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:15659234-Cercopithecus aethiops, pubmed-meshheading:15659234-Immunoprecipitation, pubmed-meshheading:15659234-In Situ Hybridization, pubmed-meshheading:15659234-Membrane Microdomains, pubmed-meshheading:15659234-Molecular Sequence Data, pubmed-meshheading:15659234-Myristic Acids, pubmed-meshheading:15659234-Neoplasm Proteins, pubmed-meshheading:15659234-Palmitic Acids, pubmed-meshheading:15659234-Protein Isoforms, pubmed-meshheading:15659234-Protein Subunits, pubmed-meshheading:15659234-Protein Transport, pubmed-meshheading:15659234-RNA, Messenger, pubmed-meshheading:15659234-Rats, pubmed-meshheading:15659234-Rats, Wistar, pubmed-meshheading:15659234-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15659234-Synapses
pubmed:year
2005
pubmed:articleTitle
BAALC 1-6-8 protein is targeted to postsynaptic lipid rafts by its N-terminal myristoylation and palmitoylation, and interacts with alpha, but not beta, subunit of Ca/calmodulin-dependent protein kinase II.
pubmed:affiliation
Department of Neuroplasticity, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't