rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-1-20
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pubmed:abstractText |
Both the astrocytic cytokine S100B and the pro-inflammatory interleukin-1 (IL-1) are elevated in Alzheimer's disease, and each has been implicated in Alzheimer-related neuropathology. We examined the gene-regulatory events through which S100B induces IL-1beta expression. In primary microglia, S100B activated the transcription factors Sp1 and NFkappaB, followed by an increase in IL-1beta mRNA levels. The latter was blocked by a peptide inhibitor of NFkappaB or by a double-stranded oligonucleotide containing a NFkappaB-binding site to serve as "decoy" DNA and reduce available NFkappaB. But in primary cortical neurons, decoy and siRNA experiments indicated that the IL-1beta induction by S100B was mediated by Sp1 without evidence of a role for NFkappaB. Our results suggest that the elevation of S100B and IL-1 in Alzheimer brain and consequent neurodegenerative events are mediated through cell-type specific gene-regulatory events, providing mechanistic insight into connections between glial activation and neuronal dysfunction.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/S-100 calcium-binding protein beta...,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
546-53
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15659225-Alzheimer Disease,
pubmed-meshheading:15659225-Animals,
pubmed-meshheading:15659225-Cells, Cultured,
pubmed-meshheading:15659225-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:15659225-Gene Expression Regulation,
pubmed-meshheading:15659225-Interleukin-1,
pubmed-meshheading:15659225-Microglia,
pubmed-meshheading:15659225-NF-kappa B,
pubmed-meshheading:15659225-Nerve Growth Factors,
pubmed-meshheading:15659225-Neurons,
pubmed-meshheading:15659225-RNA, Messenger,
pubmed-meshheading:15659225-RNA, Small Interfering,
pubmed-meshheading:15659225-Rats,
pubmed-meshheading:15659225-S100 Proteins,
pubmed-meshheading:15659225-Sp1 Transcription Factor,
pubmed-meshheading:15659225-Transcription Factors
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pubmed:year |
2005
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pubmed:articleTitle |
S100B-induced microglial and neuronal IL-1 expression is mediated by cell type-specific transcription factors.
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pubmed:affiliation |
Department of Geriatrics, University of Arkansas for Medical Sciences, 629 Jack Stephens Drive #807, Little Rock, AR 72205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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